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Stackingrig complete and first results
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microman



Joined: 14 Jan 2017
Posts: 97

PostPosted: Sun Mar 05, 2017 10:01 am    Post subject: Stackingrig complete and first results Reply with quote

Two months ago i had not been photographing for 7 years except for some snaps with the mobile. I remember i lost interest becouse photographing things i see everyday did not apeal to me. Then recently i stumbled on into reading about macro and then micro photography.

I was amazed how these microscopic beings looked magnified. I then found this forum and read alot. Started ordering things and finally its almost finished. I had no camera so i bought a Fuji X-T20 and i love it !

Got to use the rig for the first time this weekend.

Specs are.

Fuji X-T20
Wemacro rail
Nikon PB-6 bellows
Reverse Raynox 150
Olympus 10x 0.3 UIS2 objective
DIY 10W Cree LED lamp.
DIY Baseplate with 35Kg weight on. (Works very good)
Rubberbands are for keeping the rail from shifting when it screws ( also works amazingly good ! Found out about it in the forums. )

What i still need to do is mount an flocked extension between the Raynox and Objective for optimal corner sharpness and contrast ( i have read on the forum)

Also got a XYZ linear stage on the way for subject positioning. Its way to combersum to do manually....

Build one more LED lamp to get side lightning. Its only 1 direction now even with diffusor.

I still need to learn Zerene stacker. The picture below is a very small dead night butterfly. If the head looks wierd its becouse i dropped it many times trying to mount it ;D

I pushed the 10X to mayby 12x, not sure. I had problems with haloing and overexposure during stacking so i underexposed during shoot.
The picture is made from 160 separate files at 5um. Mayby a bit overkill but i didnt want to do it twice if it was too coarse.

Hopefully i can improve with training



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Saul



Joined: 31 Jan 2011
Posts: 914
Location: Naperville, IL USA

PostPosted: Sun Mar 05, 2017 10:27 am    Post subject: Reply with quote

Great result for the first stack !
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Saul
Studio, horizontal and field setups
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microman



Joined: 14 Jan 2017
Posts: 97

PostPosted: Sun Mar 05, 2017 10:36 am    Post subject: Reply with quote

Saul wrote:
Great result for the first stack !


Thanks ! I remember reading alot of your posts during the research Smile
Its mayby my fourth stack, the previus had problems so didnt save them.
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zzffnn



Joined: 22 May 2014
Posts: 1488
Location: Texas USA

PostPosted: Sun Mar 05, 2017 10:50 am    Post subject: Reply with quote

Very nice image!

Which Wemacro rail did you buy and from which eBay seller? I saw lots of them with the same name.
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Selling my inverted phase contrast 20x objective for petri dish peeking
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microman



Joined: 14 Jan 2017
Posts: 97

PostPosted: Sun Mar 05, 2017 11:00 am    Post subject: Reply with quote

zzffnn wrote:
Very nice image!

Which Wemacro rail did you buy and from which eBay seller? I saw lots of them with the same name.


Thanks !
I only know of one Wemacro rail.
Bought it from http://www.wemacro.com/

$249 plus shipping so payed around $300.

Its great for the price. The phone app works great. I like how it says how many seconds are left so i can look on the phone while its stacking.
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MacroLab3D



Joined: 31 Jan 2017
Posts: 61
Location: Ukraine

PostPosted: Sun Mar 05, 2017 11:12 am    Post subject: Reply with quote

Amazing stack. Like your lightning. Nice Ambient occlusion. Already bought few jogurts fro myself Wink preparing my system which I am also building based on info here and other places such as Flickr. Based on MP-e65. Not as close as yours - only 5x. But i will be able to go from 1X to 5X which is going to be interesting too. 2 month more i guess to finish.
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microman



Joined: 14 Jan 2017
Posts: 97

PostPosted: Sun Mar 05, 2017 11:45 am    Post subject: Reply with quote

MacroLab3D wrote:
Amazing stack. Like your lightning. Nice Ambient occlusion. Already bought few jogurts fro myself Wink preparing my system which I am also building based on info here and other places such as Flickr. Based on MP-e65. Not as close as yours - only 5x. But i will be able to go from 1X to 5X which is going to be interesting too. 2 month more i guess to finish.


Thanks Smile
The MP-E65 is great from what i have seen, then you also can use it for both studio and nature. Microscope objectives are kind of limited that way.
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microman



Joined: 14 Jan 2017
Posts: 97

PostPosted: Mon Mar 06, 2017 1:51 pm    Post subject: Reply with quote

Added a small extension between Raynox and objective that i flocked with black velour. Also flocked the RMS adapter so the backside of the objective wont reflect back. This seems to have decreased the haloing on bright surfaces If its the velour or extension i cant say but good upgrade.

It can be seen in this picture compared to the last one.

These extensions seem rare. Mayby not alot of uses for them in regular photography.

http://www.ebay.com/itm/52mm-Spacer-Ring-For-Lens-Filter-Adapter-Tube-Metal-Hood-Special-Effects-52-mm-/371761276862

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abpho



Joined: 17 Aug 2011
Posts: 1423
Location: Earth

PostPosted: Mon Mar 06, 2017 10:27 pm    Post subject: Reply with quote

Good job. You are off to a great start.
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GaryB



Joined: 29 Jul 2016
Posts: 373

PostPosted: Mon Mar 06, 2017 11:38 pm    Post subject: Reply with quote

Beautiful picture, that subtle, muted lighting is really appealing.
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microman



Joined: 14 Jan 2017
Posts: 97

PostPosted: Tue Mar 07, 2017 11:36 am    Post subject: Reply with quote

abpho wrote:
Good job. You are off to a great start.


GaryB wrote:
Beautiful picture, that subtle, muted lighting is really appealing.


Thanks guys.

Here is a picture of the new Swedish "1" Krona. Its the smallest letter on the coin. Mayby 12X

First full frame.



100% Crop. What is that yellow thing ?
I did play with Crocus pollen before, might be that or some residue from the melting of the brass.

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rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 18248
Location: Richland, Washington State, USA

PostPosted: Tue Mar 07, 2017 11:52 am    Post subject: Reply with quote

I notice that the letter is brightly lit on its right side, but the yellow thing is lit brightly on its left side.

So, if the letter is raised, then the yellow thing looks like a pit.

You might be able to quickly tell pit from bump by visual exam at high mag, seeing which way you need to adjust focus between edge and center.

--Rik
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microman



Joined: 14 Jan 2017
Posts: 97

PostPosted: Tue Mar 07, 2017 12:09 pm    Post subject: Reply with quote

rjlittlefield wrote:
I notice that the letter is brightly lit on its right side, but the yellow thing is lit brightly on its left side.

So, if the letter is raised, then the yellow thing looks like a pit.

You might be able to quickly tell pit from bump by visual exam at high mag, seeing which way you need to adjust focus between edge and center.

--Rik


Its raised. You are right, i did not think of the ligthing but now that i look at it it sure looks like a pit.

Or mayby the reflection of the bottom of the S bounces the light to the left side.
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 18248
Location: Richland, Washington State, USA

PostPosted: Tue Mar 07, 2017 2:37 pm    Post subject: Reply with quote

It could also be partially shadowed.

You would have to play with the focus to tell for sure.

The best thing to do first may be to look at the source frames in your stack. If DOF is equal or smaller than the feature height, then you should be able to tell from the image sequence whether the center is higher or lower.

--Rik
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microman



Joined: 14 Jan 2017
Posts: 97

PostPosted: Thu Mar 09, 2017 12:37 pm    Post subject: Reply with quote

rjlittlefield wrote:
It could also be partially shadowed.

You would have to play with the focus to tell for sure.

The best thing to do first may be to look at the source frames in your stack. If DOF is equal or smaller than the feature height, then you should be able to tell from the image sequence whether the center is higher or lower.

--Rik


I played around with the focus and it looks like a blob of yellow somthing.

Been testing out Bugslabber and Zerene togheter. Here is 22 pmax slabs and then dmap in Zerene. Worked pretty good. Still some haloing at some parts but that seems to be from out of focus areas that leaks into other parts. Is there some trick to avoid it ?

160 pics and still only very shallow dof.. I wonder how many pics to get whole thing in focus.




Last edited by microman on Sun Mar 12, 2017 3:07 am; edited 1 time in total
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