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coscinodiscus(?)
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Smokedaddy



Joined: 07 Oct 2006
Posts: 530
Location: Phoenix, Arizona

PostPosted: Tue Feb 14, 2017 10:48 pm    Post subject: coscinodiscus(?) Reply with quote

Just guessing on this diatom. Anyone happen to know? Perhaps a link. I wasn't able to find it in the database I was looking at.



-JW:


Last edited by Smokedaddy on Wed Feb 15, 2017 8:49 am; edited 1 time in total
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Beatsy



Joined: 05 Jul 2013
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Location: Malvern, UK

PostPosted: Wed Feb 15, 2017 1:54 am    Post subject: Reply with quote

Stephanopyxis sp.
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5641
Location: Issaquah, WA USA

PostPosted: Wed Feb 15, 2017 3:13 am    Post subject: Reply with quote

Coming along nicely. Getting some good images with your new toys!
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Smokedaddy



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PostPosted: Wed Feb 15, 2017 8:50 am    Post subject: Reply with quote

Thanks, I'm trying and enjoying it as well.
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Smokedaddy



Joined: 07 Oct 2006
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PostPosted: Wed Feb 15, 2017 2:40 pm    Post subject: Reply with quote

Another diatoms today. <g> I'm not sure how to capture the 'sphere' stacking wise. Either the objective won't resolve it properly, or I need to change some settings in Zerene. I haven't a clue how to stack any smaller increments if that's the problem. In any case at least you get the illusion of it being a sphere even though it gets less sharp as you reach the circumference.



-JW:


Last edited by Smokedaddy on Wed Feb 15, 2017 3:15 pm; edited 1 time in total
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Beatsy



Joined: 05 Jul 2013
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Location: Malvern, UK

PostPosted: Wed Feb 15, 2017 2:44 pm    Post subject: Reply with quote

The dry objective is likely the cause of the "out of focus circumference". As you focus "deeper" into the mountant, the correction for coverslip thickness drifts out of "optimum". Same happens with non correcting objectives at lower NA, just not quite as quickly.
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rjlittlefield
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Joined: 01 Aug 2006
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Location: Richland, Washington State, USA

PostPosted: Wed Feb 15, 2017 3:03 pm    Post subject: Reply with quote

If depth of mountant is the problem, then you might be able to work around it by pausing the shoot midway and re-adjusting the correction collar.

There's no guarantee this will work, however. Features along the periphery of a sphere may be seen by only about half of the objective's aperture, while the other half of the aperture sees OOF foreground. This effect can cut contrast pretty badly. It's another version of the issue diagrammed at http://www.photomacrography.net/forum/viewtopic.php?p=135042#135042 .

Quote:
Either the objective won't resolve it properly, or I need to change some settings in Zerene.

Check your source images. If source is sharp, then it make sense to think about changing settings in Zerene. If source is not sharp, Zerene cannot fix the problem.

--Rik
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Smokedaddy



Joined: 07 Oct 2006
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Location: Phoenix, Arizona

PostPosted: Wed Feb 15, 2017 3:37 pm    Post subject: Reply with quote

Thanks for the replies. On another note, has anyone tried this plugin with ImageJ?

http://bigwww.epfl.ch/deconvolution/deconvolutionlab2/

-JW:
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Smokedaddy



Joined: 07 Oct 2006
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PostPosted: Wed Feb 15, 2017 3:44 pm    Post subject: Reply with quote

This is interesting.

https://www.youtube.com/watch?v=MJ2FOFqr7hw&list=PLa9tw4-uwVLTHGoBhxHaXwVMY2ANCObHW
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Smokedaddy



Joined: 07 Oct 2006
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PostPosted: Wed Feb 15, 2017 5:13 pm    Post subject: Reply with quote

Here's another I finished today.



-JW:


Last edited by Smokedaddy on Thu Feb 16, 2017 9:52 am; edited 1 time in total
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carlos.uruguay



Joined: 23 Feb 2012
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PostPosted: Thu Feb 16, 2017 5:08 am    Post subject: Reply with quote

Very nice!
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JH



Joined: 09 Mar 2013
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Location: Vallentuna, Stockholm, Sweden

PostPosted: Thu Feb 16, 2017 1:25 pm    Post subject: Reply with quote

Nice
Regards Jörgen
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Cactusdave



Joined: 09 Jun 2009
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PostPosted: Fri Feb 17, 2017 2:39 am    Post subject: Reply with quote

Delighted to see those diatom slides being put to such productive use. Very Happy You are learning fast.
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Smokedaddy



Joined: 07 Oct 2006
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Location: Phoenix, Arizona

PostPosted: Fri Feb 17, 2017 5:49 pm    Post subject: Reply with quote

Cactusdave wrote:
Delighted to see those diatom slides being put to such productive use. Very Happy You are learning fast.


Thanks to you! I'm having a blast. I can spend 'weeks' on these puppies. I've photographed several dozen already, just haven't posted them. Didn't want to bore anyone. <g> I can't wait to figure this all out and buy a couple high-end objectives.

Regards,
-JW:

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Beatsy



Joined: 05 Jul 2013
Posts: 776
Location: Malvern, UK

PostPosted: Sat Feb 18, 2017 2:50 am    Post subject: Reply with quote

That one came out nicely, really get a sense of the 3d shape from it.
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