3D SIM Super Resolution BPAE Cells
Moderators: rjlittlefield, ChrisR, Chris S., Pau
3D SIM Super Resolution BPAE Cells
Structured Illumination Microscopy of BPAE Cells with 100X 1.49 Apo TIRF objective.
F-Actin excited at 488nm, mitochondria excited at 561nm, and the nuclei excited at 405nm; lambda ND acquisition.
I am not young enough to know everything.
- carlos.uruguay
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Very interesting!
Are you positive about the wavelength though.
F-Actin (cytoskeleton) excited at 488nm (blue)?
mitochondria excited at 561nm (green)?
and the nuclei excited at 405nm (violet)?
What is the red?
I could be wrong, but it looks to me that
Green is cytoskeleton (actin),
Blue/violet is nuclei
And red is mitochondria
Are you positive about the wavelength though.
F-Actin (cytoskeleton) excited at 488nm (blue)?
mitochondria excited at 561nm (green)?
and the nuclei excited at 405nm (violet)?
What is the red?
I could be wrong, but it looks to me that
Green is cytoskeleton (actin),
Blue/violet is nuclei
And red is mitochondria
Selling my Canon FD 200mm F/2.8 lens