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3D SIM Super Resolution BPAE Cells

 
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Choronzon



Joined: 21 Feb 2010
Posts: 370
Location: Chicago USA

PostPosted: Mon Jun 13, 2016 6:44 pm    Post subject: 3D SIM Super Resolution BPAE Cells Reply with quote




Structured Illumination Microscopy of BPAE Cells with 100X 1.49 Apo TIRF objective.
F-Actin excited at 488nm, mitochondria excited at 561nm, and the nuclei excited at 405nm; lambda ND acquisition.
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Jacek



Joined: 02 Oct 2011
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PostPosted: Mon Jun 13, 2016 11:33 pm    Post subject: Reply with quote

nice
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carlos.uruguay



Joined: 23 Feb 2012
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PostPosted: Tue Jun 14, 2016 6:10 am    Post subject: Reply with quote

Very interesting
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Pau
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Joined: 20 Jan 2010
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Location: Valencia, Spain

PostPosted: Tue Jun 14, 2016 6:33 am    Post subject: Reply with quote

Great!

Interesting technique not at the reach of us mere amateurs
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Pau
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Saul



Joined: 31 Jan 2011
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PostPosted: Tue Jun 14, 2016 7:47 am    Post subject: Reply with quote

Impressive picture. Description - even more ! Wink
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Saul
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zzffnn



Joined: 22 May 2014
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PostPosted: Tue Jun 14, 2016 10:01 am    Post subject: Reply with quote

Very interesting!

Are you positive about the wavelength though.

F-Actin (cytoskeleton) excited at 488nm (blue)?
mitochondria excited at 561nm (green)?
and the nuclei excited at 405nm (violet)?

What is the red?

I could be wrong, but it looks to me that
Green is cytoskeleton (actin),
Blue/violet is nuclei
And red is mitochondria
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Choronzon



Joined: 21 Feb 2010
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Location: Chicago USA

PostPosted: Tue Jun 14, 2016 12:48 pm    Post subject: Reply with quote

zzffnn wrote:


I could be wrong, but it looks to me that
Green is cytoskeleton (actin),
Blue/violet is nuclei
And red is mitochondria


You are correct, but those are the emission spectrum, not the excitation wavelengths.
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zzffnn



Joined: 22 May 2014
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PostPosted: Tue Jun 14, 2016 1:23 pm    Post subject: Reply with quote

^ Thank you and sorry for my brain fart Embarassed somehow the word "excited" did not register in my mind.
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Choronzon



Joined: 21 Feb 2010
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Location: Chicago USA

PostPosted: Tue Jun 14, 2016 5:51 pm    Post subject: Reply with quote

Pau wrote:
Great!

Interesting technique not at the reach of us mere amateurs


Like scanning electron microscopy, I presume?
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