3D SIM Super Resolution BPAE Cells

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Choronzon
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3D SIM Super Resolution BPAE Cells

Post by Choronzon »

Image


Structured Illumination Microscopy of BPAE Cells with 100X 1.49 Apo TIRF objective.
F-Actin excited at 488nm, mitochondria excited at 561nm, and the nuclei excited at 405nm; lambda ND acquisition.
I am not young enough to know everything.

Jacek
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Post by Jacek »

nice

carlos.uruguay
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Post by carlos.uruguay »

Very interesting

Pau
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Post by Pau »

Great!

Interesting technique not at the reach of us mere amateurs
Pau

Saul
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Post by Saul »

Impressive picture. Description - even more ! :wink:
Saul
μ-stuff

zzffnn
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Post by zzffnn »

Very interesting!

Are you positive about the wavelength though.

F-Actin (cytoskeleton) excited at 488nm (blue)?
mitochondria excited at 561nm (green)?
and the nuclei excited at 405nm (violet)?

What is the red?

I could be wrong, but it looks to me that
Green is cytoskeleton (actin),
Blue/violet is nuclei
And red is mitochondria
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Choronzon
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Location: Chicago USA

Post by Choronzon »

zzffnn wrote:
I could be wrong, but it looks to me that
Green is cytoskeleton (actin),
Blue/violet is nuclei
And red is mitochondria
You are correct, but those are the emission spectrum, not the excitation wavelengths.
I am not young enough to know everything.

zzffnn
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Post by zzffnn »

^ Thank you and sorry for my brain fart :oops: somehow the word "excited" did not register in my mind.
Selling my Canon FD 200mm F/2.8 lens

Choronzon
Posts: 384
Joined: Sun Feb 21, 2010 9:14 am
Location: Chicago USA

Post by Choronzon »

Pau wrote:Great!

Interesting technique not at the reach of us mere amateurs
Like scanning electron microscopy, I presume?
I am not young enough to know everything.

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