Lighting for macro photography of fern gametophytes
Moderators: rjlittlefield, ChrisR, Chris S., Pau
This is a single shot with the the mitutoyo mounted on a Olympus Zuiko 200mm manual prime, connected via a screw-on connector, and with the olympus connected to my canon camera via an Olympus to Canon adapter. Do You think this image should be sharper than this?
This is the Helicon stack from 52 slices, 20 microns apart each.
the setup:
Much work needed, I think.
This is the Helicon stack from 52 slices, 20 microns apart each.
the setup:
Much work needed, I think.
If I were you I'd test it at a couple of apertures, starting at wide open; single shots or short stacks would be sufficient. As I said, sometimes contrast does improve a bit on stopping down, but watch for degradation of sharpness, or vignetting. Also check if the aperture is really doing anything at all when you begin to stop it down. For many tube lenses, you can stop it down a bit and the exposure doesn't change. I think there is no harm in stopping down to that point, and it may improve contrast. Beyond that point, you should be cautious.
The objective was paid for by a university grant called "biomaker" which requires that I write down from start to finish how I did the work so that other people could do the same. My write-up is here:
https://github.com/BioMakers/23_Focus-s ... /README.md
A lot of the technical design came from this forum (http://www.photomacrography.net/) and I have put that acknowledgement right up top. I hope that comes across.
The microscope focus rail was a present from Doc.Al on this forum. He was really very kind indeed and actually brought it to my front door.
All the write-up is done now, and I just need to strap on my courage and try the setup with an actual fern. That is the tricky bit as they are quite fiddly, and tend to roll up that just the wrong moment.
https://github.com/BioMakers/23_Focus-s ... /README.md
A lot of the technical design came from this forum (http://www.photomacrography.net/) and I have put that acknowledgement right up top. I hope that comes across.
The microscope focus rail was a present from Doc.Al on this forum. He was really very kind indeed and actually brought it to my front door.
All the write-up is done now, and I just need to strap on my courage and try the setup with an actual fern. That is the tricky bit as they are quite fiddly, and tend to roll up that just the wrong moment.
Last edited by jsp on Fri Sep 22, 2017 5:22 am, edited 1 time in total.
I have four weeks left before I have to exhibit my final design in the Engineering Department at Cambridge University (UK) and I'd be delighted to have extra people on the team listing at the top, and different ideas incorporated, if anyone would like to join the fun. :-) Chris S said an acknowledgement would suit him nicely, so he's listed in the text.