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Lighting for macro photography of fern gametophytes
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jsp



Joined: 28 Mar 2015
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Location: United Kingdom

PostPosted: Mon Dec 04, 2017 11:22 pm    Post subject: Reply with quote

This is what I get with the Mity 10x:



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Chris S.
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Joined: 05 Apr 2009
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Location: Ohio, USA

PostPosted: Tue Dec 05, 2017 3:03 am    Post subject: Reply with quote

Jen,

Congrats on your grant! Exciting. Smile

I would not be quick to rule out the Mitutoyo 2x. I use my Mitty 2x all the time. Even though I have better lenses for 2x, there is huge convenience in being able to shoot a subject at 2x, 5x, 7.5x, 10x, 20x, 50x, and 100x without repositioning the macro rig, specimen, or lights. Also, there is an advantage in making these lens changes quickly, before a subject specimen dries out or moves.

In a head-to-head test at 2x, some of my other optics would test better. But none would be as convenient in the common situation just described.

Granted, the Mitty 2x has a small amount of axial CA, However, I've found this easy to remove during raw conversion.

NB, I do studio macro on an APS-C crop frame camera. I don't recall ever shooting the 2x Mitty on full frame 36x24mm. I do have the ability to do this. If someone else doesn't know already and report in, and if I don't remember, feel free to nudge me by email and I'll do a quick test for you.

Cheers,

--Chris S.

--edited typos


Last edited by Chris S. on Wed Dec 06, 2017 1:24 am; edited 1 time in total
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jsp



Joined: 28 Mar 2015
Posts: 354
Location: United Kingdom

PostPosted: Tue Dec 05, 2017 3:23 am    Post subject: Reply with quote

Hi ChrisS,

Thanks so much for looking in. I'm full of curiosity to know what the better optics are now. [smile] I wondered if you would have a minute to tell me? I'm really chasing great optics with this, and keen to not do anything daft by buying something that turns out to be second best.

I've written off-list with more information about my grant, as I'm keen to make sure full attribution is given to your Bratcam and to the others on this list.

I've to write these teaching materials, but I'm keenly aware that the information has all come from the folks on this forum and I want to make sure full attribution is given and that I get my facts right.

Thanks!
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Pau
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PostPosted: Tue Dec 05, 2017 4:47 am    Post subject: Reply with quote

Very nice images, you got it!

IMO, having the MP-E and a good microscope I would use the first one between 1X and 4X and the microscope for higher than 20X, so with the Mitty 5X and 20X you will be well served, although the convenience highlighted by Chris can be an important factor
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PostPosted: Tue Dec 05, 2017 6:20 am    Post subject: Reply with quote

Thanks Pau, that's useful to know. :-) I'm glad you like the images.
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ChrisR
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PostPosted: Tue Dec 05, 2017 7:48 am    Post subject: Reply with quote

Hmm, I don't see anything wrong with the first of the MP-E images at the scale shown, but the other two appear to be blurred. Could that be before you cracked a vibration problem?

There are loads of sharp MPE images, even a couple of mine Shocked .

There is one other reason for sticking to the Mitutoyo if you can -
SENSOR DUST! The less you remove the camera lens, the better Sad .
But I still wouldn't part with MP-E, unless I had another 1:1 macro lens.
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PostPosted: Tue Dec 05, 2017 8:03 am    Post subject: Reply with quote

That's funny. :-) The MPE images don't look right to me at all, and I know I often think images look great when others don't. I don't really know how to express what I think it wrong with them though.
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ChrisR
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PostPosted: Tue Dec 05, 2017 8:23 am    Post subject: Reply with quote

Probably a lighting issue.
The only MP-E eg I can call to mind where there's a fullish frame then a crop is this - it's sharp enough?
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PostPosted: Tue Dec 05, 2017 9:41 am    Post subject: Reply with quote

It's very nice. :-) It's quite different from my subject though isn't it? My ferns come out gritty with the MP-E and I'm not sure why, as they're fine with the Mity.
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ChrisR
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PostPosted: Tue Dec 05, 2017 11:26 am    Post subject: Reply with quote

Gritty? Confused
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PostPosted: Tue Dec 05, 2017 11:44 am    Post subject: Reply with quote

Yes mine kind of look gritty. I'm not sure why.
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PostPosted: Tue Dec 05, 2017 11:52 am    Post subject: Reply with quote

This is the 100% for the two images:

Mity 10x



MP-E at 5x

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Pitufo



Joined: 21 Jun 2015
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PostPosted: Tue Dec 05, 2017 1:52 pm    Post subject: Reply with quote

There is a Canon camera setting which allows for automatic CA correction for each lens (e.g. the MPE65). Do you have this enabled?
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PostPosted: Tue Dec 05, 2017 11:09 pm    Post subject: Reply with quote

Gosh! I'm not sure actually. I'll have a look today and see if it makes a difference. Thanks!
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ChrisR
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PostPosted: Wed Dec 06, 2017 12:11 am    Post subject: Reply with quote

Check in DPP.
--

Could this be focus banding?
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