Aspidisca

Wim van Egmond · Post by **Wim van Egmond** » Sat Feb 07, 2015 5:15 am

Some images from a couple of weeks ago I had a marine sample of diatoms scraped from the rocks and it was full of ciliates. I tried to use the higher magnifications.

The top (I think a Diophrys) was done with a 40X planapo. The second (Aspidisca) and third with a 63X planapo, the third a bit cropped.

The bottom 2 were made with a 100X planachromat. Aachromats are often regarded as inferior but with the highest magnification they give more contrast than the planapos. I removed the color fringe in lightroom. The bottom 2 are stacks. The 3D effect is not that pronounced but it is still fun to see 3D with such magnification.

Wim

fpelectronica · Post by **fpelectronica** » Sat Feb 07, 2015 5:30 am

Jacek · Post by **Jacek** » Sat Feb 07, 2015 5:38 am

Very, very nice

Marek Mis · Post by **Marek Mis** » Sat Feb 07, 2015 6:50 am

Very nice images !. The view of the internal structures is incredible.

Marek

Sumguy01 · Post by **Sumguy01** » Sat Feb 07, 2015 6:57 am

These are very good.
Thanks for sharing.

Bruce Taylor · Post by **Bruce Taylor** » Sat Feb 07, 2015 7:27 am

Superb!

zzffnn · Post by **zzffnn** » Sat Feb 07, 2015 7:38 am

Stunning photos, nice contrast and lots details!

Wim: Is this real DIC or your DIY DIC ? http://www.microscopy-uk.org.uk/mag/ind ... iydic.html If neither, Would you please provide some details of filter / set-up? Thank you!

I found circular filter stops difficult to DIY for >= 60x objectives .

In my hands, my DIY oblique+darkfield filter does not seem to perform this well at >60x (even with help of flash and EtOH). I have not tried them but doubt stacking / sharpening can make my image 1/4 as good as yours. Please kindly comment. Thanks again.

Wim van Egmond · Post by **Wim van Egmond** » Sat Feb 07, 2015 8:53 am

Thanks friends.

Zzffnn, it was done with real DIC. The Diydic oblique rheinberg filters don't work well with the highest magnifications.

Wim

Clado · Post by **Clado** » Sat Feb 07, 2015 8:56 am

Heel erg mooi, Wim.

Protos · Post by **Protos** » Sat Feb 07, 2015 9:29 am

dropping jaws !
I guess you used flash

carlos.uruguay · Post by **carlos.uruguay** » Sat Feb 07, 2015 11:56 am

Maravilloso!!!!!
Wonderful!!

arturoag75 · Post by **arturoag75** » Sat Feb 07, 2015 2:21 pm

Stunning shots Wim and great 3d effect

discomorphella · Post by **discomorphella** » Sat Feb 07, 2015 3:32 pm

Spectacular photos and a great find. I love Aspidisca and its cousins (just look at my site name). So this was just a biofilm from the rocks, not mud?

David

RogelioMoreno · Post by **RogelioMoreno** » Sun Feb 08, 2015 10:07 am

Wow, beautiful set!

Rogelio

piermicro · Post by **piermicro** » Sun Feb 08, 2015 11:54 am

Very nice .