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First 6D shot

 
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jin



Joined: 26 Aug 2012
Posts: 79
Location: Singapore

PostPosted: Tue Feb 25, 2014 6:14 am    Post subject: First 6D shot Reply with quote

After shooting many shots with 450D and notice its noise level is still high at low light area I tried shooting with 6D (my 1st attempt) with 1.4x and found noise level is much lower with compared with 450D IMHO.

White Royal butterfly egg (Pratapa deva relata)
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abpho



Joined: 17 Aug 2011
Posts: 1423
Location: Earth

PostPosted: Wed Feb 26, 2014 3:31 pm    Post subject: Reply with quote

Image looks great.
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rjlittlefield
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Joined: 01 Aug 2006
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Location: Richland, Washington State, USA

PostPosted: Wed Feb 26, 2014 4:26 pm    Post subject: Reply with quote

This is a spectacular image. The fine detail of the egg makes a really nice contrast of textures against the frothy/frilly appearance of the leaf hairs. Lighting and sharpness are excellent.

I'm thinking this doesn't really fit as initially placed, in the Beginner's forum.

Any objections if I move it to the regular Microscope gallery so that it will get the attention it deserves?

--Rik
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jin



Joined: 26 Aug 2012
Posts: 79
Location: Singapore

PostPosted: Thu Feb 27, 2014 1:51 am    Post subject: Reply with quote

abpho wrote:
Image looks great.

Hi abpho, Rik, thanks for the kind words.

Rik, I'm fine if you think this fits in to the Microscope gallery better.

Regards,
jin
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Jacek



Joined: 02 Oct 2011
Posts: 4712
Location: Poland

PostPosted: Thu Feb 27, 2014 9:46 am    Post subject: Reply with quote

Shocked
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Planapo



Joined: 07 Nov 2006
Posts: 1533
Location: Germany, in the United States of Europe

PostPosted: Thu Feb 27, 2014 10:14 am    Post subject: Reply with quote

Excellent!

Could you tell us what microscope equipment you were using and how you set-up lighting for this photo?

Quote:
... (my 1st attempt) with 1.4x ...
Was it a 1.4x objective you were using on your microscope? Or what is the "1.4x" referring to?

--Betty
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 18237
Location: Richland, Washington State, USA

PostPosted: Thu Feb 27, 2014 10:31 am    Post subject: Reply with quote

Good questions, Betty!

The caption at http://s40.photobucket.com/user/HuDie/media/Photomicrography/white-royal-6D-1024_zpscf4b3817.jpg.html (linked from the image) says this:
Quote:
180mm tube length, Nikon 10x Objective & 1.4X extension tube, 6D and 580 EX I flash. 4um step

I'm thinking the "1.4X extension tube" means a teleconverter with lenses, not an empty tube.

But I'm curious about the "180mm tube length". Does this mean an infinite objective used on combination with a 180mm converging lens? Or is it a finite objective used on 180 mm of empty extension (followed by the teleconverter)? The similar looking image at http://s40.photobucket.com/user/HuDie/media/Photomicrography/white-royal_zps11ad4cb8.jpg.html says "Nikon 10x/0.28 160mm tube lens". Now I'm really puzzled, because to my knowlege Nikon doesn't make a 10x/0.28. Mitutoyo maybe? Or Nikon 10X/0.25?

Also, now I'm wondering whether this was shot A) using a microscope body, or B) using a microscope objective added to camera as suggested in the FAQ: How can I hook a microscope objective to my camera? (If it's B then we should move the topic again, to the Macro gallery.)

Sorry for all the questions!

Can you clarify?

--Rik
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RogelioMoreno



Joined: 20 Nov 2009
Posts: 2953
Location: Panama

PostPosted: Thu Feb 27, 2014 5:18 pm    Post subject: Reply with quote

Beautiful shot!

Rogelio
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arturoag75



Joined: 05 Feb 2010
Posts: 1572
Location: italy

PostPosted: Fri Feb 28, 2014 3:23 am    Post subject: Reply with quote

Amazing shot! Shocked
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jin



Joined: 26 Aug 2012
Posts: 79
Location: Singapore

PostPosted: Fri Feb 28, 2014 4:53 am    Post subject: Reply with quote

Planapo wrote:
Excellent!

Could you tell us what microscope equipment you were using and how you set-up lighting for this photo?

Quote:
... (my 1st attempt) with 1.4x ...
Was it a 1.4x objective you were using on your microscope? Or what is the "1.4x" referring to?

--Betty


Hi Betty,

1.4x referring to a Kenko teleplus 1.4x teleconverter, see pic below for setup info, which is self explanatory :-)

Rgds,
jin
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jin



Joined: 26 Aug 2012
Posts: 79
Location: Singapore

PostPosted: Fri Feb 28, 2014 5:05 am    Post subject: Reply with quote

Hi Rik,

Sorry for the confusion, I should be more accurate with my caption, figure were not with my mind when I typed :-)

See pic at above for setup and equipment used for shooting the eggs and correction of captions:

Equipment used for egg pic in this post:
180mm tube length (from shoulder of objective to CCD), Nikon 10X/0.25, 160/- WD5.6 objective &amp and 1.4X extension tube Teleplus Pro 300 teleconverter, Canon 6D and 580EX I flash, @4um step.

Similar looking egg in photobucket:
Nikon 10x/0.25 160/- WD5.6 160mm tube (shoulder of obj. to CCD), 4um step. Canon 450D + 580EX I flash.

Rgds,
jin
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Marek Mis



Joined: 10 Jul 2010
Posts: 2442
Location: Suwalki, Poland

PostPosted: Fri Feb 28, 2014 5:07 am    Post subject: Reply with quote

Very impresive image !

Marek
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rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 18237
Location: Richland, Washington State, USA

PostPosted: Fri Feb 28, 2014 12:11 pm    Post subject: Reply with quote

Great description -- thanks for the info!

--Rik
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Ken Ramos



Joined: 27 Jul 2006
Posts: 6996
Location: lat=35.4005&lon=-81.9841

PostPosted: Wed Apr 02, 2014 6:31 am    Post subject: Reply with quote

You know, these days when I look at things such as this I often wonder, just how does the DNA know to code for such an elaborate design such as this? One can speculate over many reasons for the design of course but knowing just how the A's, T's, C's, and G's all get placed in the right sequence to produce this beautiful design both amazes and baffles me at the same time. I got a lot of reading up and studying to do and of course most all of it will be over my head but if I don't make the attempt, it will stay there ... over my head. Laughing

Wonderful image and well taken. Congrats!
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johan



Joined: 06 Sep 2011
Posts: 964

PostPosted: Wed Apr 02, 2014 6:54 am    Post subject: Reply with quote

Good choice for photo of the month, it's such a beautiful image!
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