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Fruit Fly

 
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pwnell



Joined: 18 Dec 2009
Posts: 1911
Location: Tsawwassen, Canada

PostPosted: Thu Sep 13, 2012 7:19 pm    Post subject: Fruit Fly Reply with quote

I like red wine. A lot. So what do you do as macro photographer when fruit flies invade this peaceful tranquility? You catch them and photograph them of course!

I had some issues with a deep stack on the second portrait. The antennae did not stack properly. With Rik's help I managed to get a better stack. Both are ZereneStacker PMax, and taken at 10x (the second one has an additional 1.6x magnification). Oh if the sharpening is a bit too much blame Flickr. The second stack is 701 images deep Surprised


20120910-DSLR_IMG_0001_1.jpg by pwnell, on Flickr


20120911-DSLR_IMG_0234-Edit.jpg by pwnell, on Flickr
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 18000
Location: Richland, Washington State, USA

PostPosted: Thu Sep 13, 2012 8:21 pm    Post subject: Reply with quote

Excellent -- this came out great! That 10X NA 0.40 sure is sharp, but the wide aperture means deep stacks.

I know you had to un-check all those alignment parameters and do a Stack Selected on the antennae, but I'm curious to know exactly how you did the final merging of antennae over the eyes. Was that done with the Photoshop layers with masks and Darken/Lighten mode, or did you come up with some better way to do it?

--Rik
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pwnell



Joined: 18 Dec 2009
Posts: 1911
Location: Tsawwassen, Canada

PostPosted: Thu Sep 13, 2012 8:37 pm    Post subject: Reply with quote

rjlittlefield wrote:
I know you had to un-check all those alignment parameters and do a Stack Selected on the antennae, but I'm curious to know exactly how you did the final merging of antennae over the eyes. Was that done with the Photoshop layers with masks and Darken/Lighten mode, or did you come up with some better way to do it?

--Rik

I'd love to know how to do the darken/lighten cloning, but I don't. I used photoshop to merge the two layers, by using a layer mask at 400x and picking out the antenna by hand.
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 18000
Location: Richland, Washington State, USA

PostPosted: Thu Sep 13, 2012 8:52 pm    Post subject: Reply with quote

That works. The single mask is more labor intensive, but it can make a better result too.

--Rik
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Jacek



Joined: 02 Oct 2011
Posts: 4577
Location: Poland

PostPosted: Fri Sep 14, 2012 4:33 am    Post subject: Reply with quote

excellent photos
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5713
Location: Issaquah, WA USA

PostPosted: Fri Sep 14, 2012 12:08 pm    Post subject: Reply with quote

Waldo,

Excellent work!

With only 3mm of working distance I am curious, how did you illuminated these?
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pwnell



Joined: 18 Dec 2009
Posts: 1911
Location: Tsawwassen, Canada

PostPosted: Fri Sep 14, 2012 12:31 pm    Post subject: Reply with quote

Charles Krebs wrote:
Waldo,

Excellent work!

With only 3mm of working distance I am curious, how did you illuminated these?


I used this:



Very unsophisticated. Basically three Ikea lamps to the back, left and right of the objective. The objective is wrapped inside a paper cylinder (shaded image). This extends to below the subject.

It is very hard to work like this because I cannot see anything. My first subject was bumped off the pin because I drove the objective into the pin Sad

PS: Exposure times were ISO200 and 1/8 so long exposures were needed.
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Marek Mis



Joined: 10 Jul 2010
Posts: 2397
Location: Suwalki, Poland

PostPosted: Wed Sep 19, 2012 1:53 pm    Post subject: Reply with quote

Waldo,

Excellent and beautiful photomicrographs !

Marek
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