My new microscope

[pwnell]

Yesterday I received my Olympus BX53 DIC microscope after many months of research, asking questions on this forum, reviewing demos and finally taking the (expensive) plunge. Now it is up to me to learn how to get the best from this microscope.

Here are some random photos I took today. Comments / critique welcome.

Copepod nauplii, DIC, 40x


Barnacle stack (50 photos, Helicon method A I think), DIC, 20x


Barnacle, Polarized, 20x


Barnacle, Polarized, 4x (not aesthetically pleasing but interesting nonetheless)


Rotifer, 40x, DIC


No idea what this is. DIC, 40x


Here is a video clip of above (63MB): http://www.waldonell.com/images/20120728-MVI_0082.mp4

Smaller one (8.5MB): http://www.waldonell.com/images/20120728-MVI_0082-2.mp4

[Litonotus]

congratulations (: I'm sure we will see world class images soon (:
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I'm looking for CFI PlanApo objectives: 4, 10, 60A/1,4, and Plan Fluor 100x/0,5-1,3 if you see a listing at a reasonable price or have any for sale let me know.

[Pau]

Top quality pictures from great optics...
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Pau

[Cactusdave]

You are fortunate indeed to have such a great instrument and I'm sure it will give you a huge amount of pleasure. Your pictures are already very good. Learning to 'fine tune' the DIC settings is always good advice for someone with a new DIC-equipped microscope to play with.
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Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

[pwnell]

Thanks for your comments. The biggest thing I am struggling with at the moment is getting even illumination across the field of view. To get the maximum DIC contrast I adjust the slider, and stop before the view goes black. But just before it goes black and a bit before that point, the illumination is uneven.

How do you prevent this? Currently I am fixing it in post but it is a pain.

[arturoag75]

Congratulations,
it's a really stunning scope and great shot yours ones!
what optics ? fluorite?
best
arturo
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www.microscopeitaly.it

[pwnell]

[Cactusdave]

Obtaining even illumination across the field is the major headache for all DIC applications. The difficulty in giving precise advice is that all manufacturers implement DIC in rather different ways. In general it is necessary to carefully independently adjust whatever variable settings are available both before the light enters the objective, including the diaphragm and also those available after the specimen, before the light enters the eyepiece. If you are not familiar with it, this article may be helpful http://www.olympusmicro.com/primer/techniques/dic/dicconfiguration.html read in conjunction with the specific instructions you should have for DIC on the BX53.
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Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

[RogelioMoreno]

Waldo,

Congratulations.

Nice pictures with an excellent instrument. Please show us pictures of that beautiful instrument.

Rogelio

[pwnell]

Here is a quick one... Pardon the annotations - I did that for someone.

[Litonotus]

that is a great scope (:

fluorescence module?
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I'm looking for CFI PlanApo objectives: 4, 10, 60A/1,4, and Plan Fluor 100x/0,5-1,3 if you see a listing at a reasonable price or have any for sale let me know.

[pwnell]

[RogelioMoreno]

Wow, beautiful scope.

Rogelio

[pwnell]

[Jacek]

Wonderful microscope. You're in luck