Tulip once again

bernhardinho · Post by **bernhardinho** » Sun Jan 28, 2007 8:02 am

Hi folks,

but this time I tried Astrablue/Safranin/Chrydoisin. Makes nice colors:

Nice set of trichomes:

Bernhard

Ken Ramos · Post by **Ken Ramos** » Sun Jan 28, 2007 9:17 am

You out did yourself here Bernhard.

Tell us, what sort of microtome are you using to cut these, show us a photo? "Inquiring minds want to know" (TV commercial here stateside).

I must say the stains have produced some very striking colorful effects and I also must say you are pioneering the way here on the forums for more images like these. Look forward to seeing more.

beetleman · Post by **beetleman** » Sun Jan 28, 2007 2:47 pm

Now that is one beautiful Picture Bernhard. Simply fantastic colors and forms.

bernhardinho · Post by **bernhardinho** » Sun Jan 28, 2007 2:49 pm

No problem, your honour!!

it's very similar to what you've ordered. The bladeholder uses dispensable microtomeblades by Leica (My taste is easy, always the best

)
Sometimes I use a simple barber's shaving knife (or whatever you call them!)

Bernhard

Ken Ramos · Post by **Ken Ramos** » Sun Jan 28, 2007 2:59 pm

Ah, so this is the tool that helps to make those fantastic images possible. Hope mine will be just as useful when it arrives. Normally I am like you, I too have simple tastes if at all possible but there is the age old adage, "you get what you pay for." So, for a $30.00US microtome, I am not expecting a whole lot.

Thanks Bernhard

bernhardinho · Post by **bernhardinho** » Sun Jan 28, 2007 3:29 pm

Ken Ramos wrote:" So, for a $30.00US microtome, I am not expecting a whole lot.

I'm very positive about that! All that gimmick actually does, is get hold of the specimen and lift it a tiny bit. All the rest depends very much on the sharpness of the blade you use, and the preparation of the thing you cut ( to make it an easy cut) and lots of practice of course.

Bernhard

gpmatthews · Post by **gpmatthews** » Sun Jan 28, 2007 6:14 pm

The pictures are wonderful. I must admit I am tempted, but so many things to try and so little time!

bernhardinho · Post by **bernhardinho** » Mon Jan 29, 2007 4:26 am

Hi folks,

for anybody willing to try the combination of dyes, I have to admit a major faux-pas ( probably old age induced lack of concentration!) : the combi should be Astrablue/FUCHSIN/Chrysoidin. The Chrysoidin was intended to replace Safranin. Just to avoid spreading nonsense in the web (there is plenty of already).

Apologies

Bernhard

bernhardinho · Post by **bernhardinho** » Mon Jan 29, 2007 4:30 am

gpmatthews wrote:The pictures are wonderful. I must admit I am tempted, but so many things to try and so little time!

Thanks Graham,

it's easier than it looks. The whole process including making the pics took about an hour!!

Bernhard

Charles Krebs · Post by **Charles Krebs** » Mon Jan 29, 2007 9:56 am

Bernhard... really nice!

Did you make the bladeholder, or can they be purchased? I have a very similar hand microtome, but have been frustrated trying to use the razor that came with it. The disposable blades are not too expensive, but I have not yet found a reasonably priced holder for them. What angle is the blade held at when cutting?

Walter Piorkowski · Post by **Walter Piorkowski** » Mon Jan 29, 2007 11:32 am

Excellent work Bernhard.
Walt

Bruce Williams · Post by **Bruce Williams** » Tue Jan 30, 2007 10:09 am

Beautiful work Bernhard - has almost a three dimensional look to it. Colours are stunning!

Bruce