Bacteria-map
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Bacteria-map
on the surface of freshwater samples often occurs a flower of bacteria.
Under the fluorescence microscope with blue excitation and
stained with Acridin-Orange the masses of bacteria form a
map-like arrangement of cells with different fluorescence colors.
Magnification about 1000x.
Thanks for looking,
Gerd
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Thanks all for the kind comments.
@ bromodomain: you can determine living from dead bacteria cells,
if it is the same genus in the sample and if you have constant, known pH conditions.
In this case, living cell have green, dead ones red fluorescence.
In a water sample you normally have lots of different bacteria and you do not know
the pH conditions. The emitted colour spectrum can be very different, depending on the genus.
So i think, the red ones are a different genus from the green ones in this sample.
@Franz: yes, i put some coverglasses onto the water surface and picked them
again to put them onto a slide with the stain.
Thanks,
Gerd
@ bromodomain: you can determine living from dead bacteria cells,
if it is the same genus in the sample and if you have constant, known pH conditions.
In this case, living cell have green, dead ones red fluorescence.
In a water sample you normally have lots of different bacteria and you do not know
the pH conditions. The emitted colour spectrum can be very different, depending on the genus.
So i think, the red ones are a different genus from the green ones in this sample.
@Franz: yes, i put some coverglasses onto the water surface and picked them
again to put them onto a slide with the stain.
Thanks,
Gerd
This is very interesting indeed! I recently tried the same experiment at home with my microscope. At first everything was looking a little blurry, and I was able to make everything out. Then I put on my contact lenses, and suddenly there it was clear as day! I had not realized until then how important my contacts really are to me. Thank you very much for sharing this, the photos look great!