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Spirostomum - a huge ciliate protozoan (+ an onion cell)

 
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NikonUser



Joined: 04 Sep 2008
Posts: 2530
Location: southern New Brunswick, Canada

PostPosted: Wed Mar 09, 2011 7:52 am    Post subject: Spirostomum - a huge ciliate protozoan (+ an onion cell) Reply with quote

These guys are currently the dominant species in my overwintering pond water (in the garage). Almost too dense to photograph with transmitted light; also very active.
Anterior end to the right; beaded nucleus; striated surface; short cilia all over the surface.
Olympus 10x SPlan achro.+ 1.25x intermediate lens + 2.5x relay lens; DIC.
The scale is a micrometer slide photographed at same scope setting.



NU11016 NU11017 NU11018
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NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives


Last edited by NikonUser on Thu Mar 10, 2011 12:06 pm; edited 1 time in total
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Mitch640



Joined: 15 Aug 2010
Posts: 2137

PostPosted: Thu Mar 10, 2011 9:22 am    Post subject: Reply with quote

Very nice details NU. I really like the effect of DIC. It can show so much more than regular brightfield.
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NikonUser



Joined: 04 Sep 2008
Posts: 2530
Location: southern New Brunswick, Canada

PostPosted: Thu Mar 10, 2011 12:05 pm    Post subject: Reply with quote

It's a pity that DIC prisms are hard to find, and once found are often prohibitively expensive. Under 'regular' transmitted light these beasts are featureless, you just seel a body with lots of small circles (probably food storage particles). I just love looking at small 'animacules' under DIC.

DIC also works well with plant cells. A recent article
HERE
describes how to see onion cells' nuclei using a stain. With DIC you can look at live cells and see the nuclei and the cytoplasm streaming within the cell (the lines crossing the cell):

NU11019
_________________
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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Mitch640



Joined: 15 Aug 2010
Posts: 2137

PostPosted: Thu Mar 10, 2011 12:15 pm    Post subject: Reply with quote

I can look at these images all day. They contain so much information that is easy to understand once you see it. I just wish my old scope could do it. LOL
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5761
Location: Issaquah, WA USA

PostPosted: Thu Mar 10, 2011 10:04 pm    Post subject: Reply with quote

Very nice shots! Great view of the macronucleus, especially that last image.
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NikonUser



Joined: 04 Sep 2008
Posts: 2530
Location: southern New Brunswick, Canada

PostPosted: Fri Mar 11, 2011 7:07 am    Post subject: Reply with quote

Thanks Charles. I'm still not getting the clarity and quality you are getting Sad
_________________
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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View user's profile Send private message
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