www.photomacrography.net :: View topic - I have just chanced upon this..the microscope of your dreams
www.photomacrography.net Forum Index
An online community dedicated to the practices of photomacrography, close-up and macro photography, and photomicrography.
Photomacrography Front Page Amateurmicrography Front Page
Old Forums/Galleries
 
 FAQFAQ   SearchSearch   MemberlistMemberlist   UsergroupsUsergroups   RegisterRegister 
 ProfileProfile   Log in to check your private messagesLog in to check your private messages   Log inLog in 
I have just chanced upon this..the microscope of your dreams

 
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Macro & Microscopy Articles
View previous topic :: View next topic  
Author Message
sonyalpha



Joined: 24 Apr 2010
Posts: 916
Location: Middle England

PostPosted: Tue Oct 05, 2010 11:41 am    Post subject: I have just chanced upon this..the microscope of your dreams Reply with quote

Browsing the web for particular insects I chanced upon this.......I wonder if it has been highlighted before?

http://www.dailymail.co.uk/sciencetech/article-1256658/Creepy-crawlies-close-Insects-magnified-million-times-size.html

Amazing stuff:

sonyalpha
_________________
Retired but not old in spirit:

Fairly new to photography........keen to learn:
Back to top
View user's profile Send private message Visit poster's website
rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 18244
Location: Richland, Washington State, USA

PostPosted: Tue Oct 05, 2010 12:32 pm    Post subject: Reply with quote

I don't recall if that particular article has been linked before, but Scanning Electron Microscopy is a standard way to look at very small stuff and is periodically discussed in the forum. See HERE, for example.

Compared to optical techniques, SEM offers the advantage of much higher resolution and DOF, at the cost of high expense and often more elaborate specimen prep requirements. Also it makes pictures that start off as grayscale. Those nice colors you see in published SEM pictures were hand-tinted by a human, based on recognizing the morphology of features seen on the subject.

--Rik
Back to top
View user's profile Send private message Visit poster's website
georgetsmurf



Joined: 30 Nov 2010
Posts: 46

PostPosted: Thu Jan 06, 2011 7:46 am    Post subject: Re: I have just chanced upon this..the microscope of your dr Reply with quote

From a scientific perspective the Electron Micrographs are brilliant as they have seemingly unlimited depth of field and the resolution is superb however as a descriptive tool they tend to fall short in my opinion as the images they produce do not look like real living creatures. Thats just my opinion. Both systems have their place I guess.
Back to top
View user's profile Send private message
enricosavazzi



Joined: 21 Nov 2009
Posts: 854
Location: Stockholm, Sweden

PostPosted: Mon Feb 14, 2011 8:18 am    Post subject: Reply with quote

I used SEM pictures in many cases, but as already mentioned in this thread they are originally monochrome images (although some false color can be added by automated processing, based on how the subject scatters electrons). It is legitimate to manually add coloring to make it easier to distinguish e.g. anatomical features, but coloring a SEM image to make it look like an optical macrograph is rather futile (in a scientific sense).

A further, important limitation of SEM imaging is that the subject is usually metal-sputtered. The resulting images are rich in surface detail but completely lack any transparency/translucency. Mirror reflections are also completely absent, so (optically) very shiny surfaces are rendered by a SEM as completely opaque, dull and featureless. All in all, an extremely useful scientific tool, but it should not be forgotten that this is a completely different technique from optical photomacro/micrography.

Of course, SEM imaging shines at resolutions that exceed those of optical imaging. Many older SEMs are unable to accommodate a field of view larger than a couple of mm, or require the specimen stage to be disassembled and reassembled at a higher distance from the objective, which usually allows a wider FOV but does not permit operation at high magnification. On a SEM I used several years ago, reassembling the stage for low magnification work and recalibrating the scope required close to half an hour. Modern machines are usually better in this respect.
_________________
--ES
Back to top
View user's profile Send private message Visit poster's website
rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 18244
Location: Richland, Washington State, USA

PostPosted: Mon Feb 14, 2011 8:40 am    Post subject: Reply with quote

enricosavazzi wrote:
A further, important limitation of SEM imaging is that the subject is usually metal-sputtered. The resulting images are rich in surface detail but completely lack any transparency/translucency. Mirror reflections are also completely absent, so (optically) very shiny surfaces are rendered by a SEM as completely opaque, dull and featureless.

Some newer scopes do not require coatings. As discussed at http://en.wikipedia.org/wiki/Environmental_scanning_electron_microscope,
"The environmental scanning electron microscope or ESEM is a scanning electron microscope (SEM) that allows for the option of collecting electron micrographs of specimens that are "wet," uncoated, or both by allowing for a gaseous environment in the specimen chamber."

However, this does not resolve any of the issues mentioned by Enrico. SEM intrinsically works through interaction of electrons with the surface of a structure -- no coherent reflections and no significant penetration -- so shininess and transparency (to light) don't get rendered even by environmental SEMs.

Some directly comparable optical and SEM images with an uncoated specimen are shown HERE.

--Rik
Back to top
View user's profile Send private message Visit poster's website
shrek



Joined: 01 Nov 2009
Posts: 98
Location: Toulouse (France)

PostPosted: Mon Feb 14, 2011 9:30 am    Post subject: Reply with quote

One interessing of SEM (MEB) is ,whith a spécial soft to déterminate the nature of a minéral ;Is me dream!

jp
Back to top
View user's profile Send private message
Display posts from previous:   
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Macro & Microscopy Articles All times are GMT - 7 Hours
Page 1 of 1

 
Jump to:  
You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot vote in polls in this forum


Powered by phpBB © 2001, 2005 phpBB Group