Yesterday I managed to buy two new "used" lenses. The first one is a 16x Plan Neofluar Imm Phase 2 with a NA of 0.50. The other one is a 63x Plan Apochromat Phase 3 with a NA of 1.40. I need to practice with the 63x as I am a rookie when it comes to oil immersion. However, here are the first shots, no further image processing.
16x Plan Neofluar Imm, Oil Immersion, Nikon D5000.
63x Plan Apochromat, Oil Immersion, Nikon D5000.
Best regards
Eckhard
Haematococcus pluvialis - more pictures added
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Haematococcus pluvialis - more pictures added
Last edited by Ecki on Sun Oct 03, 2010 9:39 am, edited 1 time in total.
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- Posts: 747
- Joined: Wed Jan 14, 2009 11:59 am
- Location: Italy
Franz,
I had the same association - modern art - when I looked at this picture. It is brightfield and not phase contrast, sorry for any confusion.
Haematococcus pluvialis can be found in puddles, bird baths, etc. This Algae loves hard water and can live even in brackish water. When environmental conditions become bad, Haematococcus pluvialis recycles its two flagellums, produces astaxanthin and morphs into an akinete. The astaxanthin is probably there to protect the akinete from the sunlight.
When environmental conditions improve, the akinete "wakes up" and morphs into the flagellated form.
Akinetes:
10x Planapo, Darkfield
100x PlanNeo, DIC
Flagellated form:
63x Planapo, Phase Contrast
63x Planapo, Phase Contrast
100x PlanNeo, DIC
Best regards
Ecki
I had the same association - modern art - when I looked at this picture. It is brightfield and not phase contrast, sorry for any confusion.
Haematococcus pluvialis can be found in puddles, bird baths, etc. This Algae loves hard water and can live even in brackish water. When environmental conditions become bad, Haematococcus pluvialis recycles its two flagellums, produces astaxanthin and morphs into an akinete. The astaxanthin is probably there to protect the akinete from the sunlight.
When environmental conditions improve, the akinete "wakes up" and morphs into the flagellated form.
Akinetes:
10x Planapo, Darkfield
100x PlanNeo, DIC
Flagellated form:
63x Planapo, Phase Contrast
63x Planapo, Phase Contrast
100x PlanNeo, DIC
Best regards
Ecki
- Cactusdave
- Posts: 1631
- Joined: Tue Jun 09, 2009 12:40 pm
- Location: Bromley, Kent, UK
You are very lucky to have acquired the 63x Plan Apochromat NA 1.40. It is a lens I used a lot when working professionaly with confocal immunofluorescence. I think it is one of the finest finite lenses Zeiss ever made. Unfortunately. like a lot of older Zeiss lenses they are prone to delamination, especially if they have been blasted with lots of intense UV from high intensity arc lamps for fluorescence applications. I'd love to own a nice one myself sometime.
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear
As you can see in the next picture something is eating my Haematococcus cysts.
63x PlanApochromat Phase Contrast
It took me a while to catch the parasite "in flagranti", but here you go:
63x PlanApochromat DIC
It is a fungus. The cyst in the middle of the right side has been infected and is eaten alive.
@Dave,
these are infinity corrected optics. I have two 63 Planapos, the Phase version and the DIC version. Both have been bought used and show no sign of delamination.
Regards
Eckhard
63x PlanApochromat Phase Contrast
It took me a while to catch the parasite "in flagranti", but here you go:
63x PlanApochromat DIC
It is a fungus. The cyst in the middle of the right side has been infected and is eaten alive.
@Dave,
these are infinity corrected optics. I have two 63 Planapos, the Phase version and the DIC version. Both have been bought used and show no sign of delamination.
Regards
Eckhard