A variety of pics from my first 'serious' photo session with the new setup. No stacking here, just more practice and fiddling with the oblique patch.
Small clover, 2x obj
I never realised the complexity of a clover:
First remotely decent 40x pic (well, more like 500x or so into the sensor, yes I'll need to use Charles' tips on measuring..)
Liked the colours in this one:
Apple, 40x obj, you can see the fibres of the flesh. Gawd, I feel so ignorant compared to you biologists!
Now I'm happy
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Now I'm happy
Simon W
EOS 5D Mk 3; Olympus BH-2; Zerene Stacker
Melbourne, Australia
EOS 5D Mk 3; Olympus BH-2; Zerene Stacker
Melbourne, Australia
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- Posts: 156
- Joined: Sun Jan 02, 2011 11:50 am
- Charles Krebs
- Posts: 5865
- Joined: Tue Aug 01, 2006 8:02 pm
- Location: Issaquah, WA USA
- Contact:
Simon,
I strongly encourage everyone to play around with oblique illumination. The specimens you show here are very thick, and have great contrast by themselves. You will see a difference from "straight" brightfield, but it may not be that significant. The real value from oblique comes more when you work with subjects that are thinner, relatively "clear", and have a refractive index closer to the medium where they exist. You will see this if/when you get into the tiny water critters.
I strongly encourage everyone to play around with oblique illumination. The specimens you show here are very thick, and have great contrast by themselves. You will see a difference from "straight" brightfield, but it may not be that significant. The real value from oblique comes more when you work with subjects that are thinner, relatively "clear", and have a refractive index closer to the medium where they exist. You will see this if/when you get into the tiny water critters.