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The subtleties of fly ID's - stereo pair added
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NikonUser



Joined: 04 Sep 2008
Posts: 2528
Location: southern New Brunswick, Canada

PostPosted: Tue Mar 09, 2010 6:43 am    Post subject: The subtleties of fly ID's - stereo pair added Reply with quote

A couple of warm days brought out a few overwintering flies including this small (6 mm) specimen.
A member of the House fly family (Muscidae), genus Caricea.

Final key characters for this genus:
1: 2 pairs of reclinate orbital bristles, the anterior pair situated at
about the middle of the frons and well before level of anterior ocellus.
2:frons with 2 pairs of inclinate frontal bristles.


Head shot: Nikon 4x Plan Achro objective (at 7.1x), 81 frames @ 0.01 mm, ZS PMax


_________________
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives


Last edited by NikonUser on Tue Mar 16, 2010 3:03 am; edited 3 times in total
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Barry



Joined: 18 Jan 2009
Posts: 146
Location: Netherlands

PostPosted: Tue Mar 09, 2010 7:29 am    Post subject: Reply with quote

Very nice images - with which objective was the top one shot; Micro-nikkor 105/4?

Best wishes,
Barry
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Eric F



Joined: 11 Nov 2008
Posts: 246
Location: Sacramento, Calif.

PostPosted: Tue Mar 09, 2010 9:08 am    Post subject: Reply with quote

Absolutely fantastic fly photos NU!

Eric
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NikonUser



Joined: 04 Sep 2008
Posts: 2528
Location: southern New Brunswick, Canada

PostPosted: Tue Mar 09, 2010 9:26 am    Post subject: Reply with quote

Thanks, Barry and Eric.

Full lateral is with a Micro Nikon 105MF 2.8 @ f/8 on lens; 33 frames @ 0.1 mm. ZS PMax.

Both with styrofoam cup diffuser/single flash.
_________________
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5713
Location: Issaquah, WA USA

PostPosted: Tue Mar 09, 2010 11:47 am    Post subject: Reply with quote

NU,

These look great! Wonderful details!
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NikonUser



Joined: 04 Sep 2008
Posts: 2528
Location: southern New Brunswick, Canada

PostPosted: Tue Mar 09, 2010 6:11 pm    Post subject: Reply with quote

Thanks Charles. Good to see some bug life after 4 months of Winter.
Counting bristles is a bit easier than dissecting genitalia but the latter may be needed to get to the species level.
_________________
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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LordV



Joined: 22 Nov 2007
Posts: 1561
Location: UK

PostPosted: Tue Mar 09, 2010 11:57 pm    Post subject: Reply with quote

Wonderful stacks !
Have to admit i gave up on definitive ID's for flies when told I would have to dissect out the testicles to be certain Smile
Brian V.
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canon20D,350D,40D,5Dmk2, sigma 105mm EX, Tamron 90mm, canon MPE-65
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RogelioMoreno



Joined: 20 Nov 2009
Posts: 2953
Location: Panama

PostPosted: Wed Mar 10, 2010 4:26 am    Post subject: Reply with quote

Beautiful images, excellent details.

Rogelio
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NikonUser



Joined: 04 Sep 2008
Posts: 2528
Location: southern New Brunswick, Canada

PostPosted: Sat Mar 13, 2010 4:28 am    Post subject: Reply with quote

Thanks Brian & Rogelio.

Brian: 'Dissecting genitalia' is a bit of a misnomer. The interesting bits in males are really external but they tend to tuck them into the abdomen (modesty?).

I find the variation in the detail, between species, fascinating. Why would otherwise identical looking species, particularly in males, show such differences in external genitalia?

They also make great macro subjects.

left image: Nikon 20x microscope objective on bellows, 38 frames @ 0.005 mm; ZS PMax
right image: Nikon 10x microscope objective on bellows, 28 frames@0.01 mm; ZS PMax.

NU10-007
_________________
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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booter



Joined: 25 Feb 2010
Posts: 21
Location: Illinois

PostPosted: Sat Mar 13, 2010 10:17 am    Post subject: Reply with quote

Chaetotaxy made fun! Great images NU.

Scott
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rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 18001
Location: Richland, Washington State, USA

PostPosted: Sat Mar 13, 2010 11:47 am    Post subject: Reply with quote

I haven't spent much time staring at fly genitals, but this one seems similar to some butterflies.

The structures are quite complex.

Any chance of getting that right side structure in stereo?

--Rik
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NikonUser



Joined: 04 Sep 2008
Posts: 2528
Location: southern New Brunswick, Canada

PostPosted: Sun Mar 14, 2010 7:10 am    Post subject: Reply with quote

Unlike moth genitalia which can be flattened into practically 2-dimensions, some fly genitalia are more like spheres with all sorts of bits and pieces. They probably are best seen as stereo pairs. however, I have never been able to see anything but 2 separate images when looking at stereo pairs.
_________________
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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View user's profile Send private message
rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 18001
Location: Richland, Washington State, USA

PostPosted: Sun Mar 14, 2010 8:54 pm    Post subject: Reply with quote

Thanks to NU's generosity and the miracle of email, here are a couple of synthetic 3D views of this genitalia.

Rocking animations:



Stereo pair (crossed eye):



NU tells me this was a wet mount, in glycerine with no cover glass. Hairs sticking out of the surface layer of glycerine show up as very bright highlights. Those disrupt the off-axis views that we need for stereo. The rotating versions are straight out of PMax (except for levels adjustment and animation in ImageReady), but I had to do some manual cleanup in Photoshop to get the stereo pair that you see here.

This is a good test set -- interesting enough to get me excited, but troublesome enough to prompt some future mods to the software.

If I can get something that NU can see in stereo, now that'll be cool!

--Rik
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LordV



Joined: 22 Nov 2007
Posts: 1561
Location: UK

PostPosted: Mon Mar 15, 2010 12:03 am    Post subject: Reply with quote

NikonUser wrote:
Unlike moth genitalia which can be flattened into practically 2-dimensions, some fly genitalia are more like spheres with all sorts of bits and pieces. They probably are best seen as stereo pairs. however, I have never been able to see anything but 2 separate images when looking at stereo pairs.


NU re- 3-d cross-eye stereograms -have you tried sitting about 2 feet away from the screen and then holding a pencil or finger about 4" in front of your nose and focusing on that. If you can do that you should see a new image in the middle of the stereo pair. It's then a matter of trying to slide the finger/pencil out of the way whilst keeping the third image in view. With a bit of brain training you learn to lock this third image.
Brian V.
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canon20D,350D,40D,5Dmk2, sigma 105mm EX, Tamron 90mm, canon MPE-65
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NikonUser



Joined: 04 Sep 2008
Posts: 2528
Location: southern New Brunswick, Canada

PostPosted: Tue Mar 16, 2010 3:02 am    Post subject: Reply with quote

Brian:
Making slow progress. The pencil trick gives me 3 out of focus images images (4 counting the pencil), and a temporary head ache. As I slide the pencil away the 3rd middle bug image disappears with it.
Could be my brain is too old to train.
_________________
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
Back to top
View user's profile Send private message
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