Fresh mount in water on cavity slide
10x Nikon CF N Plan objective, 47 frames @ 0.004mm, ZS PMax stack
NU09247
Freshwater Cyclops
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Freshwater Cyclops
Ventral view, from my local roadside ditch.
Fresh mount in water on cavity slide
10x Nikon CF N Plan objective, 47 frames @ 0.004mm, ZS PMax stack
NU09247
Fresh mount in water on cavity slide
10x Nikon CF N Plan objective, 47 frames @ 0.004mm, ZS PMax stack
NU09247
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
-
Cactusdave
- Posts: 1631
- Joined: Tue Jun 09, 2009 12:40 pm
- Location: Bromley, Kent, UK
Chris:
Yes, coverslip for both; seems to produce less reflection than a water surface. I can recommend 'pond-dipping' as a means to get a whole bunch of stuff. One extremely rich site in the 1950's was the canal near Woking station; Horsell Common was a good spot for insects.
Cactusdave:
Sedated with alcohol.
Yes, coverslip for both; seems to produce less reflection than a water surface. I can recommend 'pond-dipping' as a means to get a whole bunch of stuff. One extremely rich site in the 1950's was the canal near Woking station; Horsell Common was a good spot for insects.
Cactusdave:
Sedated with alcohol.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
Yes, both Cyclops and Daphnia are just visible with the naked eye. Place your sample in a white-bottomed tray, about 1-2" of water. Both move with a jerky movement. Daphnia often occur in swarms and are visible in the pond as a dense cloud.
Easiest to see them with a low power stereoscope (say 3-5x); suck them up with an eye dropper pipette and 'squirt' them onto a cavity slide.
Easiest to see them with a low power stereoscope (say 3-5x); suck them up with an eye dropper pipette and 'squirt' them onto a cavity slide.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
-
Cactusdave
- Posts: 1631
- Joined: Tue Jun 09, 2009 12:40 pm
- Location: Bromley, Kent, UK
to 
........... without warning!-
augusthouse
- Posts: 1195
- Joined: Sat Sep 16, 2006 1:39 am
- Location: New South Wales Australia
Nice work NU!
This post and the alcohol approach brings to mind Charlie's bubble wrapped mite:
http://www.photomacrography.net/forum/v ... 98&start=0
Craig
This post and the alcohol approach brings to mind Charlie's bubble wrapped mite:
http://www.photomacrography.net/forum/v ... 98&start=0
Craig
To use a classic quote from 'Antz' - "I almost know exactly what I'm doing!"
The 'trick' with alcohol is little and often (I'm talking here for sedating aquatics 
)
Possibly the best way is to have your specimen in a small amount of shallow water and place it in a sealed container having a large surface area of alcohol. The alcohol should evaporate and slowly diffuse into the water giving a sedated bug. You need patience for this.
Another way, and the one I used for the Cyclops, is to add minute droplets of alcohol to the water+bug on the cavity slide at intervals of a few minutes. Cactusdave's description is perfect.
I use 99% Isopropyl Alcohol (chemist's rubbing alcohol).
)Possibly the best way is to have your specimen in a small amount of shallow water and place it in a sealed container having a large surface area of alcohol. The alcohol should evaporate and slowly diffuse into the water giving a sedated bug. You need patience for this.
Another way, and the one I used for the Cyclops, is to add minute droplets of alcohol to the water+bug on the cavity slide at intervals of a few minutes. Cactusdave's description is perfect.
I use 99% Isopropyl Alcohol (chemist's rubbing alcohol).
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives