My latest Euclanis (Rotifer) pics

Images made through a microscope. All subject types.

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Bruce Williams
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My latest Euclanis (Rotifer) pics

Post by Bruce Williams »

Hi Folks,

Forgive me for blowing my own trumpet but ... well I'm really pleased with these photos (he says preening himself). I think they are my best attempt yet and just what I needed follow a week of disappointing results.

I used the technique suggested by CK (within PeglegOS's posting "Water Critter's Technique") of slowly removing water from under the coverslip until the subject is imobilised but still alive and natural looking. As far as I know the animal was still alive when I washed the slide off.

Pic1 was taken with the 20X achro objective and the foot section(pic3) was taken with the 40X. The original images were 3264 x 2448 pixels. I did get some pics with the cilia nice and sharp (but head out of focus) however the shape of the head had changed too much (through movement) and I found it impossible to convincingly stack the 2 images (even manually in Photoshop).

What I am particularly pleased with in pic1 is the amound of subtle detail showing "through" the body of the creature. Pic2 shows what I think is muscle fibre? The foot (pic3) also shows some intriguing anatomical detail - again I only wish I had an understanding of the associated physiology :? .

Bruce

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Walter Piorkowski
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Post by Walter Piorkowski »

Very nice indeed Bruce.

Walt

beetleman
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Post by beetleman »

You bet Bruce, some great detail in these photos. Good job :smt023
Take Nothing but Pictures--Leave Nothing but Footprints.
Doug Breda

gpmatthews
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Post by gpmatthews »

Excellent phtos - and, yes, that looks like striped muscle.
Graham

Though we lean upon the same balustrade, the colours of the mountain are different.

Bruce Williams
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Joined: Mon Oct 30, 2006 1:41 pm
Location: Northamptonshire, England
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Post by Bruce Williams »

Thanks for your comments guys. I realise of course that I have just started out on this journey but it is exciting when you feel you've made a step forward.

Bruce

bernhardinho
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Post by bernhardinho »

Hi Bruce,

excellent results!! Congratulations!!

As far as english literature is concerned, I can recommend:

A Key to British Freshwater Planctonic Rotifera by Rosalind Pontin

A lot to learn there.


Keep it up!!

Bernhard

Charles Krebs
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Post by Charles Krebs »

Bruce... you should be quite pleased, you did an excellent job recording great detail from head to toe (and inside too!).

Bruce Williams
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Joined: Mon Oct 30, 2006 1:41 pm
Location: Northamptonshire, England
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Post by Bruce Williams »

Thanks for your generous comments Bernhard and Charlie - praise from you guys is praise indeed!

Thanks too for the suggested reading Bernhard - I have ordered a copy of the book and hope to be reading it by the weekend.

Bruce

gpmatthews
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Joined: Thu Aug 03, 2006 10:54 am
Location: Horsham, W. Sussex, UK
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Post by gpmatthews »

As far as english literature is concerned, I can recommend:

A Key to British Freshwater Planctonic Rotifera by Rosalind Pontin
- ye gods!!! have you seen the Amazon UK price for this at £116+, when at the FBA it is only £8.00! (see http://www.fba.org.uk/)
Graham

Though we lean upon the same balustrade, the colours of the mountain are different.

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