Askenasia volvox

Bernd · Joined: 16 Aug 2006 Posts: 46

Dear all:

Today I would like to introduce Askenasia volvox, an egg-shaped ciliate belonging to the family Didiniidae, which I found in a puddle. His mouth is surrounded by three girdles of cilia. The frontal girdle consists of motile feeding cilia. The middle girdle consists of about 60 stiff, immobile, sickle-shaped cilia, which are curving backwards, parallel to the body. These middle cilia produce the saltatory locomotion typical of this ciliate. Behind the middle girdle of cilia follows a girdle of long stiff bristles. The posterior end of the cell is unciliated. When looking along the longitudinal axis, which is possible during the frequent rest periods of this otherwise very fast swimmer, the middle girdle of cilia forms a graceful frame surrounding the animal. The long stiff bristles, which are diffucult to discern under rightfield illumination, then project through the middle cilia. Depending on the focal plane and whether the front or back end of the ciliate is directed towards the observer, very different views result.

A: Lateral view; B, C: Frontal view; D – F: View from behind.

All photographs were taken with a Canon Powershot G5 camera through a Plan-Neofluar 40x/0,75 objective attached to a Zeiss Axioskop, using brightfield illumination.

Bernd

bernhardinho · Joined: 13 Aug 2006 Posts: 506 Location: Germany

Bravo Bernd,

some fantastic shots of a very interesting organism!!

Thanks!!

Bernhard

Ken Ramos · Posted: Sun Oct 29, 2006 3:11 pm Post subject:

A beautiful set of images in the composite Bernd and I noticed a scale bar there in the corner (I am going to get difficult here Laughing

). Charlie, a few days back suggested that a scale bar would be nice to have in one of my images, figuring out anything having to do with numbers has me screaming, waving my hands and heading for the door, sort of like tying size 32 midges, so without me having to breakout a sliderule or something, could you explain how you know that little line represents 10um in relation to the organism photographed and presented? I mean could someone say just...put any line in that corner, no matter how short or how long and say it represents 10um's? I would not know the difference one way or another. In laymans terms explain please. Very Happy

Yeah, I am baiting Rik Laughing

_________________
Ken Ramos
Rutherford Co., Western North Carolina

beetleman · Posted: Sun Oct 29, 2006 4:14 pm Post subject:

Simply amazing Bernd. It is incredible how this creature could look so different in the shots. Nice work here and thank you for all the info also. Wink

_________________
Take Nothing but Pictures--Leave Nothing but Footprints.
Doug Breda

Walter Piorkowski · Joined: 14 Aug 2006 Posts: 545 Location: South Beloit, Ill

Beautiful brightfield images Bernd.

Walt

Charles Krebs · Joined: 01 Aug 2006 Posts: 4072 Location: Issaquah, WA USA

Bernd... very nice images of a fascinating subject. I've only seen two of these, and really enjoyed watching them.

Ken... I've been away for a while, and I see the scale bar issue is still on your mind. I think I will start a new subject over in in the microscope "beginners" section. It might be useful to have something on this in the forum, even if folks don't care to use them too often.

Ken Ramos · Posted: Fri Nov 03, 2006 3:41 am Post subject:

Charlie replied:

Cyclops · Posted: Fri Nov 03, 2006 10:31 am Post subject:

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