Two Paramecium bursaria saying goodbye to each other

piotr · Post by **piotr** » Fri Feb 20, 2009 7:19 pm

Final moments of binary fission of Paramecium bursaria. The cells are connected just by a thin cytoplasmic bridge as shown in the inset in the first picture.

Olympus BHT, 40x objective, DIC, Nikon D50, flash illumination

Charles Krebs · Post by **Charles Krebs** » Fri Feb 20, 2009 8:27 pm

Piotr,

Very nice! I really like the first shot.
(little dark on my monitor)

piotr · Post by **piotr** » Fri Feb 20, 2009 9:01 pm

Charlie,

Thanks! I remember your images of a similar scene from over four years ago (time flows...)

Yes, I think I exaggerated contrast curve while processing these images. This one looks more natural:

Piotr

Wim van Egmond · Post by **Wim van Egmond** » Tue Feb 24, 2009 2:37 am

Wonderful Piotr, a bit like Rodin's 'the kiss'.

I also see some dust on the sensor. It is a real nuisance that with micrographs the dust is realy 'projected' on the sensor. I have never found a way to create a dustmap automatically so I made one myself by creating a file with a saved selection of all the dots. Than I made a photoshop action that imported that selection and used it to remove the dust (with median, than adding a bit of noise and a little fading and blurring)

But if someone else has a better solution?

Wim