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Stentor

 
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gpmatthews



Joined: 03 Aug 2006
Posts: 1028
Location: Horsham, W. Sussex, UK

PostPosted: Sat Oct 14, 2006 11:09 am    Post subject: Stentor Reply with quote



Microscope: Zeiss Standard GFL
Objective: Leitz 40/0.7 NPL Fluotar ICT
Ocular: Olympus P15
Substage: Leitz ICT
DIC
Camera: Canon Powershot S50
ISO200, F5, Flash

This is a composite of 3 images stitched together: the stentor was too big for a single field shot.
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Graham

Though we lean upon the same balustrade, the colours of the mountain are different.
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Ken Ramos



Joined: 27 Jul 2006
Posts: 7058
Location: lat=35.4005&lon=-81.9841

PostPosted: Sat Oct 14, 2006 11:26 am    Post subject: Reply with quote

Graham your images are getting better everytime you post. Surprised This is a great shot of the stentor. Your DIC set up is working extremely well, I noticed the adoral zone of the stenor is very well captured along with all the other cell inclusions and vacuoles. Looks as though you are up and running with the "big guys." Wink
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Cyclops



Joined: 05 Aug 2006
Posts: 2968
Location: North East of England

PostPosted: Sat Oct 14, 2006 11:50 am    Post subject: Reply with quote

Very nice shot, great detail!
BTW, what is DIC?
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Canon 30D | Canon IXUS 265HS | Cosina 100mm f3.5 macro | EF 75-300 f4.5-5.6 USM III | EF 50 f1.8 II | Slik 88 tripod | Apex Practicioner monocular microscope
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gpmatthews



Joined: 03 Aug 2006
Posts: 1028
Location: Horsham, W. Sussex, UK

PostPosted: Sat Oct 14, 2006 12:00 pm    Post subject: Reply with quote

DIC is Differential Interference Contrast, a fancy system of illumination that increases contrast in the image by interference techniques. It produces a false 3D effect in the final image.

See

http://micro.magnet.fsu.edu/primer/techniques/dic/dichome.html

for more info.
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Graham

Though we lean upon the same balustrade, the colours of the mountain are different.
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bernhardinho



Joined: 13 Aug 2006
Posts: 560
Location: Germany

PostPosted: Sun Oct 15, 2006 5:13 am    Post subject: Reply with quote

Absolutly fantastic shot!! I love it!!

Bernhard
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5800
Location: Issaquah, WA USA

PostPosted: Sun Oct 15, 2006 4:03 pm    Post subject: Reply with quote

Graham... both this image and the vorticella you recently posted seem to show amazing depth-of-field for DIC. Very nice indeed. They carry a great deal of detail and information.
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