www.photomacrography.net :: View topic - help!, what is this bug!
www.photomacrography.net Forum Index
An online community dedicated to the practices of photomacrography, close-up and macro photography, and photomicrography.
Photomacrography Front Page Amateurmicrography Front Page
Old Forums/Galleries
 
 FAQFAQ   SearchSearch   MemberlistMemberlist   UsergroupsUsergroups   RegisterRegister 
 ProfileProfile   Log in to check your private messagesLog in to check your private messages   Log inLog in 
help!, what is this bug!

 
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope
View previous topic :: View next topic  
Author Message
Adrian



Joined: 24 Aug 2006
Posts: 191
Location: Australia

PostPosted: Fri Oct 06, 2006 2:19 am    Post subject: help!, what is this bug! Reply with quote

i found this bug in some sea water sample, its been under the slide for over 3 weeks now, and still living, they are fairly large, much larger then then ciliate, and they seem to have about 5 weird tenticale leg things on the bottom side of there body, which they seem to use as swimming device



the pic is under 40x objective with brightfeild lighting, i ajusted the levels/curves and then a unsharp mask, with photoshop

i think i need to learn how to do backround subtraction, anyone have any tips?

thanks
Back to top
View user's profile Send private message Visit poster's website MSN Messenger
bernhardinho



Joined: 13 Aug 2006
Posts: 560
Location: Germany

PostPosted: Fri Oct 06, 2006 2:36 am    Post subject: Reply with quote

Hi Adrian,

this "bug" is clearly a ciliate!! The "weird legs" are called cirri. They're sort of merged cilies and are a typical feature of the hypotrich ciliates. I don't find it particurlarly large, by the way. That looks familiar to me in 400x mag. By the shape it could be Euplotes, Aspidisca or even something like Discomorphella or Saprodinium.

As to the background: do you know this tool:

http://micro.magnet.fsu.edu/primer/digitalimaging/backgroundsubtractiontoolkit.html

It is easy to use and quite useful.

Best wishes

Bernhard
Back to top
View user's profile Send private message Visit poster's website
Ken Ramos



Joined: 27 Jul 2006
Posts: 7052
Location: lat=35.4005&lon=-81.9841

PostPosted: Fri Oct 06, 2006 3:16 am    Post subject: Reply with quote

It does look like some sort of Euplote. Very Happy
Back to top
View user's profile Send private message
beetleman



Joined: 04 Aug 2006
Posts: 3578
Location: Southern New Hampshire USA

PostPosted: Fri Oct 06, 2006 5:35 am    Post subject: Reply with quote

I would love to do a little plankton dipping
_________________
Take Nothing but Pictures--Leave Nothing but Footprints.
Doug Breda
Back to top
View user's profile Send private message Send e-mail
Wim van Egmond



Joined: 15 Aug 2006
Posts: 825
Location: Berkel en Rodenrijs, the Netherlands

PostPosted: Fri Oct 06, 2006 7:17 am    Post subject: Reply with quote

Gee, that is a good tool, Bernhard! As always it is only for PC users Sad

Wim
Back to top
View user's profile Send private message Visit poster's website
discomorphella



Joined: 01 Oct 2006
Posts: 597
Location: NW USA

PostPosted: Fri Oct 06, 2006 11:50 am    Post subject: Reply with quote

You may also want to check out the background subtraction options in imageJ. I am not too familiar with marine ciliates, but it could be something like an Aspidisca or a Discomorphella... I hope its the latter...they do like to live in fairly anoxic environments.

--David
Back to top
View user's profile Send private message
Adrian



Joined: 24 Aug 2006
Posts: 191
Location: Australia

PostPosted: Fri Oct 06, 2006 1:12 pm    Post subject: Reply with quote

oh its a ciliate? its a strange thing isnt it! well i think it is

thank you

how do i load a user defined backround for the toolkit?

ill check out that ImageJ too thanks.


i got more haha!

what are these bugs?




these are allso still alive and in the same slide as the beast tenticale ciliate, they about half the size or smaller, then the beasts, not as ugly eather.

there where about 6 different types of bugs in the orginal sea water sample, all of them have died off after the first week, accept the beast ciliates and this kind are still alive (week 3 now).

i think i did quite well processing this image, for a microphotography newbie that is!



and this one was allso in the same water however it was found on the 1st day and it was the only one i found of its kind, it vanished quite quickly though, and i never saw it again,

larger the the beasts, not as ugly and allso not as fast/agressive as them, movement and actions slow like a slug, determining the front of its body is to the left by the way it moved, strechy head area, that contracts


by the way anyone have any tips of better improving my image processing? i am just adjusting the levels/curves and unsharp mask, with photoshop

thanks
Back to top
View user's profile Send private message Visit poster's website MSN Messenger
bernhardinho



Joined: 13 Aug 2006
Posts: 560
Location: Germany

PostPosted: Sat Oct 07, 2006 4:02 am    Post subject: Reply with quote

Hi again,

well, the upper one is very likely to be an Euplotes. The lower one is a ciliate too, could be some sort of Litonotus, Amphileptus or Loxophyllum. By the way, in ciliates there is no "front side" as such. We distinguish between dorsal and ventral side, the latter being defined as to where the mouthopening (peristome) lies.

To the toolkit: well; I work with it using my intuition, I must confess. Load a picture with a more or less even background, now use the little dots you have to move around and you see the effect in the right window. When it's seems even enough click on "File/save background". From then on, everytime you load an image, you just click on "substraction image" and the saved background is apllied.

As to the image processing with PS I think there are better experts than me, but I guess it will be hard to give any advice as long as one doesn't know the original image.

Bernhard
Back to top
View user's profile Send private message Visit poster's website
Display posts from previous:   
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope All times are GMT - 7 Hours
Page 1 of 1

 
Jump to:  
You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot vote in polls in this forum


Powered by phpBB © 2001, 2005 phpBB Group