Shooting the "Carpet beetle again"

A forum to ask questions, post setups, and generally discuss anything having to do with photomacrography and photomicroscopy.

Moderators: Pau, rjlittlefield, ChrisR, Chris S.

rjlittlefield
Site Admin
Posts: 21278
Joined: Tue Aug 01, 2006 8:34 am
Location: Richland, Washington State, USA
Contact:

Shooting the "Carpet beetle again"

Post by rjlittlefield »

Image

Image

OK, I relented and took a picture of the setup I used to shoot the unmounted carpet beetle.

Some old-timers will notice that I've disconnected the illuminator stand from the stacking unit. That resolved some worries about vibration, and also simplified tipping up the stacking unit to various angles.

Despite appearances, the system is comfortably stable in this configuration. Anyone wanting to do something similar should note that all the parts in the camera chain are fastened at multiple points several inches apart.

BTW, the chunk of glass is actually two microscope slides stuck together. They came out of the box that way, refused to come apart easily, and finally I realized the double thickness could be handy by moving the specimen farther away from the balsa surface.

--Rik

nto
Posts: 181
Joined: Fri Feb 22, 2008 12:56 pm
Location: Italy

Post by nto »

Thanks Rik for Illustration, Moving micro using basis iron? or bellows?
Antonio

rjlittlefield
Site Admin
Posts: 21278
Joined: Tue Aug 01, 2006 8:34 am
Location: Richland, Washington State, USA
Contact:

Post by rjlittlefield »

Base iron. The handle at very top.

Steps needed for microscope objective are much too small to do with bellows.

0.0002 inches = 0.005 mm = 5 microns !

This is 1/5 of one tick mark on the dial, 100 tick marks per full turn.

--Rik

Danny
Posts: 725
Joined: Fri Feb 02, 2007 11:07 pm
Location: New Zealand

Post by Danny »

Excellent to see Rik and I love the lighting, excellent !!!!. Always hoping to see breathing subjects though. Another me thing I guess :wink:

All the best and great to see the setups. Never stop with these ideas, please. We need a place in the articles for ALL these setups in here. Its a wealth of information Rik.

Danny.
Worry about the image that comes out of the box, rather than the box itself.

augusthouse
Posts: 1195
Joined: Sat Sep 16, 2006 1:39 am
Location: New South Wales Australia

Post by augusthouse »

Thanks for that Rik,
It clearly demonstrates just how adaptable your setup is and opens up all sorts of other options (not that you aren't aware of them), ie, you could slide your microscope in under the bellows for photomicrography. There is also the option for integrating elaborate back-lighting methods with regard to the stage area for photomacrography; or tip it up into horizontal mode when necessary or preferred.

I like it!!

Did you mark the additional 'tick marks' on the dial by shallow engraving or with a surface method?

Craig
Last edited by augusthouse on Fri Mar 07, 2008 12:55 pm, edited 2 times in total.
To use a classic quote from 'Antz' - "I almost know exactly what I'm doing!"

rjlittlefield
Site Admin
Posts: 21278
Joined: Tue Aug 01, 2006 8:34 am
Location: Richland, Washington State, USA
Contact:

Post by rjlittlefield »

nzmacro wrote:Always hoping to see breathing subjects though.
Me too. But I haven't figured out how to pull that off except for a very few subjects -- eggs, of course, and pupae, and some caterpillars, and a moth or two, one very cooperative spider, some anaesthetized critters, and so on.

If I ever do figure out how to do it in general, rest assured that you'll hear about it --- right after the patent is filed and the product sales sheets are ready! :D
Did you mark the additional 'tick marks' on the dial by shallow engraving or with a surface method?
Neither. The dials come engraved at 100 ticks per full turn. In an earlier version, I carefully made a vernier scale on paper and taped it next to the dial. In theory, that would let me read to 1/10 of an existing tick mark. In practice, the added scale was more trouble than it was worth, because I could not reliably make movements fine enough to justify it. Now I just eyeball 1/5 of a tick or whatever I need. The uncertainty in reading the dial is comparable to variability in making the movements. For a 10X NA 0.30 objective, microscopyu calculates a DOF of about 7.5 microns. Using a nominal step size of 5 microns seems to work out OK -- no doubt I end up shooting more frames than I need, but it's not hugely more, and I don't have any trouble with missing frames.

--Rik

acerola
Posts: 251
Joined: Wed Dec 13, 2006 2:28 pm
Location: Hungary
Contact:

Post by acerola »

Rik, How can you fix the microscope objective on a bellows? They all (?) have RMS thread as I think. Any idea? (apart from making a hole on a body cap :) )
Péter

Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Post by Charles Krebs »


acerola
Posts: 251
Joined: Wed Dec 13, 2006 2:28 pm
Location: Hungary
Contact:

Post by acerola »

Thanks Charles, I ordered it.
Péter

Post Reply Previous topicNext topic