This is fun stuff. The crazy colors are due to a WHITE BALANCE button in the TOUPVIEW software that comes up with these crazy colors. At times, I use a gray card to balance the scope, but I find these variations quite dynamic.
VIVID VS1184G2
NEC D789064
Cross-polarized
ICs with a 100X oil objective
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ICs with a 100X oil objective
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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- Posts: 5090
- Joined: Sun Jan 15, 2012 12:31 pm
100X Oil
No cover slip - oil directly on the chip. When I frame the shot the image is very murky, but when I add the oil and get the meniscus the contrast jumps dramatically.
I save my chips on slides, and after my sessions I clean them in xylol.
Mike
I save my chips on slides, and after my sessions I clean them in xylol.
Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
I wonder if you might get even more contrast/sharpness with a coverslip too. I think I remember your 100x oil being a 160/0.17 - so the coverslip is designed in. Even if the oil has a very close RI to the glass, it should make a difference. Perhaps a thin film of oil between the coverslip and the chip too - just to keep it as homogenous as possible.
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- Posts: 5090
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Cover slip
Beatsy,
I'll give it a try, but the major thing that scares microscopists aout oil immersion is the excessive cleanup. I have worked out a very efficient cleanup system, so we'll see how it goes.
Mike
I'll give it a try, but the major thing that scares microscopists aout oil immersion is the excessive cleanup. I have worked out a very efficient cleanup system, so we'll see how it goes.
Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA