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Jacek

Joined: 02 Oct 2011 Posts: 5181 Location: Poland
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Harald
Joined: 13 May 2011 Posts: 497 Location: Steinberg, Norway
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Posted: Wed Jan 09, 2019 1:12 pm Post subject: |
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Hi Jacek,
Great set of images as always.
Love the 3rd one of the moquito, amazing details and light.
Thanks for sharing  _________________ Kind Regards
Harald
Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11 |
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MASZEK
Joined: 04 Jul 2018 Posts: 54 Location: POLAND
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Posted: Wed Jan 09, 2019 4:03 pm Post subject: |
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Hi Jacek.
My whole comment: great photos.
All.  |
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anne
Joined: 05 Dec 2014 Posts: 79
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Posted: Thu Jan 10, 2019 12:17 am Post subject: |
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Absolutely stunning images!
Thank you for sharing.
anne |
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santiago

Joined: 25 Sep 2018 Posts: 108 Location: Nijmegen, The Netherlands
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Posted: Thu Jan 10, 2019 5:22 am Post subject: |
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Amazing images! _________________ Santiago
Flickr |
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Lou Jost
Joined: 04 Sep 2015 Posts: 3176 Location: Ecuador
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Posted: Thu Jan 10, 2019 5:28 am Post subject: |
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These are such beautiful photos. I only wish that you would always include some minimal information about the techniques and objectives/lenses for each of these inspiring images. _________________ Lou Jost
www.ecomingafoundation.wordpress.com
www.loujost.com |
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viktor j nilsson
Joined: 01 Mar 2013 Posts: 103 Location: Lund, Sweden
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Posted: Thu Jan 10, 2019 8:08 am Post subject: |
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Just... wow. I agree with Lou, I'd really appreciate at least a brief note about the objectives and illumination for these images. In the meantime, I drool. |
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Jacek

Joined: 02 Oct 2011 Posts: 5181 Location: Poland
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Posted: Thu Jan 10, 2019 10:59 am Post subject: |
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Thank you very much
EPI (two Ikea lights + diffuser)
1. EPI + Pol (dia) (Zeiss Apo 6.3/20)
2. Pol (Zeiss Apo 6.3/20)
3. EPI (Nikon CFN PlanApo 10/0.45)
4. Pol (Nikon CFN PlanApo 10/0.45)
5. autoFL (UV) (Nikon CFN PlanApo 40/0.95)
6. autoFL (UV) (Zeiss Apo 6.3/20)
7. EPI (Nikon CFN Plan 4/0.13)
Next set:
1. BF (Nikon CFN PlanApo 10/0.45)
2. BF (Zeiss Apo 6.3/20)
3. autoFL (UV) (Zeiss Apo 6.3/20)
4. EPI (Nikon CFN PlanApo 10/0.45)
5. EPI (Zeiss Apo 6.3/20)
6. DIC (Nikon CFN Plan Fluor 40/0.75)
7. DIC (Nikon CFN Plan Fluor 40/0.75)
 _________________ https://photos.app.goo.gl/MV9IynUtBdaKkVj53 |
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Lou Jost
Joined: 04 Sep 2015 Posts: 3176 Location: Ecuador
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ChrisR Site Admin
Joined: 14 Mar 2009 Posts: 8161 Location: Near London, UK
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Posted: Thu Jan 10, 2019 11:35 am Post subject: |
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Super pictures, again
I believe you are using afocal method, yes?
When you change from Zeiss to Nikon, do you change eyepieces?
I apologize if you have already covered this! _________________ Chris R |
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Jacek

Joined: 02 Oct 2011 Posts: 5181 Location: Poland
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Posted: Thu Jan 10, 2019 12:30 pm Post subject: |
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Yes, I'm still doing it afokal, I do not change my glasses.
I have Chinese glasses 10X/20 with a rejected pupil.
I use Nikon CFWN 10X/20 glasses only for DIC.
 _________________ https://photos.app.goo.gl/MV9IynUtBdaKkVj53 |
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pbraub
Joined: 02 Feb 2018 Posts: 34
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Posted: Thu Jan 10, 2019 12:40 pm Post subject: |
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Spectacular images, thank you Jacek!
What lightsource do you use for UV excitation? Do you excite through the objective or from the side?
Kind regards
Peter |
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Robert Berdan

Joined: 18 Oct 2017 Posts: 97 Location: Calgary
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Posted: Thu Jan 10, 2019 9:55 pm Post subject: Amazing Photomicrographs |
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Exceptional images Jacek you are an inspiration. Well done.
Rob |
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Sumguy01
Joined: 28 Jan 2013 Posts: 1245 Location: Ketchikan Alaska USA
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Posted: Thu Jan 10, 2019 11:49 pm Post subject: |
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Excellent photos.
Thanks for sharing. |
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Jacek

Joined: 02 Oct 2011 Posts: 5181 Location: Poland
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Posted: Fri Jan 11, 2019 12:43 am Post subject: |
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Thanks
I use fluorescence on two microscopes. On one FL epi (LED lighting 3W 365 nm (UV) and 460 nm (blue)).
On the second one I have FL dia, one diode 365 nm on it, a converted condenser, enlarging the diode + UG5 filter and barrier filter.
In addition, for small magnifications (10X lens and small) I use two illuminators that I have made (365 LEDs + UG5 excitation filters) - this solution is very efficient lighting.
 _________________ https://photos.app.goo.gl/MV9IynUtBdaKkVj53 |
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