Various

Jacek · Joined: 02 Oct 2011 Posts: 5325 Location: Poland

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https://photos.app.goo.gl/MV9IynUtBdaKkVj53

Harald · Joined: 13 May 2011 Posts: 555 Location: Steinberg, Norway

Hi Jacek,
Great set of images as always.
Love the 3rd one of the moquito, amazing details and light.

Thanks for sharing Very Happy

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Kind Regards
Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11

MASZEK · Joined: 04 Jul 2018 Posts: 67 Location: POLAND

Hi Jacek.
My whole comment: great photos.
All. Very Happy

anne · Joined: 05 Dec 2014 Posts: 133

Absolutely stunning images!
Thank you for sharing.
anne

santiago · Posted: Thu Jan 10, 2019 5:22 am Post subject:

Amazing images!
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Santiago
Flickr

Lou Jost · Joined: 04 Sep 2015 Posts: 3615 Location: Ecuador

These are such beautiful photos. I only wish that you would always include some minimal information about the techniques and objectives/lenses for each of these inspiring images.
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Lou Jost
www.ecomingafoundation.wordpress.com
www.loujost.com

viktor j nilsson · Joined: 01 Mar 2013 Posts: 116 Location: Lund, Sweden

Just... wow. I agree with Lou, I'd really appreciate at least a brief note about the objectives and illumination for these images. In the meantime, I drool.

Jacek · Joined: 02 Oct 2011 Posts: 5325 Location: Poland

Thank you very much Smile

EPI (two Ikea lights + diffuser)

1. EPI + Pol (dia) (Zeiss Apo 6.3/20)
2. Pol (Zeiss Apo 6.3/20)
3. EPI (Nikon CFN PlanApo 10/0.45)
4. Pol (Nikon CFN PlanApo 10/0.45)
5. autoFL (UV) (Nikon CFN PlanApo 40/0.95)
6. autoFL (UV) (Zeiss Apo 6.3/20)
7. EPI (Nikon CFN Plan 4/0.13)

Next set:

1. BF (Nikon CFN PlanApo 10/0.45)
2. BF (Zeiss Apo 6.3/20)
3. autoFL (UV) (Zeiss Apo 6.3/20)
4. EPI (Nikon CFN PlanApo 10/0.45)
5. EPI (Zeiss Apo 6.3/20)
6. DIC (Nikon CFN Plan Fluor 40/0.75)
7. DIC (Nikon CFN Plan Fluor 40/0.75)

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https://photos.app.goo.gl/MV9IynUtBdaKkVj53

Lou Jost · Joined: 04 Sep 2015 Posts: 3615 Location: Ecuador

Thanks very much for the added information and for the second set! It is much appreciated.
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Lou Jost
www.ecomingafoundation.wordpress.com
www.loujost.com

ChrisR · Posted: Thu Jan 10, 2019 11:35 am Post subject:

Super pictures, again Smile

I believe you are using afocal method, yes?
When you change from Zeiss to Nikon, do you change eyepieces?

I apologize if you have already covered this!
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Chris R

Jacek · Joined: 02 Oct 2011 Posts: 5325 Location: Poland

Yes, I'm still doing it afokal, I do not change my glasses.
I have Chinese glasses 10X/20 with a rejected pupil.
I use Nikon CFWN 10X/20 glasses only for DIC.

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pbraub · Joined: 02 Feb 2018 Posts: 57

Spectacular images, thank you Jacek!

What lightsource do you use for UV excitation? Do you excite through the objective or from the side?

Kind regards
Peter

Robert Berdan · Joined: 18 Oct 2017 Posts: 143 Location: Calgary

Exceptional images Jacek you are an inspiration. Well done.
Rob

Sumguy01 · Posted: Thu Jan 10, 2019 11:49 pm Post subject:

Excellent photos.
Thanks for sharing.

Jacek · Joined: 02 Oct 2011 Posts: 5325 Location: Poland

Thanks

I use fluorescence on two microscopes. On one FL epi (LED lighting 3W 365 nm (UV) and 460 nm (blue)).
On the second one I have FL dia, one diode 365 nm on it, a converted condenser, enlarging the diode + UG5 filter and barrier filter.
In addition, for small magnifications (10X lens and small) I use two illuminators that I have made (365 LEDs + UG5 excitation filters) - this solution is very efficient lighting.

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