Various

Jacek · Post by **Jacek** » Wed Jan 09, 2019 12:47 pm

Harald · Post by **Harald** » Wed Jan 09, 2019 1:12 pm

Hi Jacek,
Great set of images as always.
Love the 3rd one of the moquito, amazing details and light.

Thanks for sharing

MASZEK · Post by **MASZEK** » Wed Jan 09, 2019 4:03 pm

Hi Jacek.
My whole comment: great photos.
All.

anne · Post by **anne** » Thu Jan 10, 2019 12:17 am

Absolutely stunning images!
Thank you for sharing.
anne

santiago · Post by **santiago** » Thu Jan 10, 2019 5:22 am

Amazing images!

Lou Jost · Post by **Lou Jost** » Thu Jan 10, 2019 5:28 am

These are such beautiful photos. I only wish that you would always include some minimal information about the techniques and objectives/lenses for each of these inspiring images.

viktor j nilsson · Post by **viktor j nilsson** » Thu Jan 10, 2019 8:08 am

Just... wow. I agree with Lou, I'd really appreciate at least a brief note about the objectives and illumination for these images. In the meantime, I drool.

Jacek · Post by **Jacek** » Thu Jan 10, 2019 10:59 am

Thank you very much

EPI (two Ikea lights + diffuser)

1. EPI + Pol (dia) (Zeiss Apo 6.3/20)
2. Pol (Zeiss Apo 6.3/20)
3. EPI (Nikon CFN PlanApo 10/0.45)
4. Pol (Nikon CFN PlanApo 10/0.45)
5. autoFL (UV) (Nikon CFN PlanApo 40/0.95)
6. autoFL (UV) (Zeiss Apo 6.3/20)
7. EPI (Nikon CFN Plan 4/0.13)

Next set:

1. BF (Nikon CFN PlanApo 10/0.45)
2. BF (Zeiss Apo 6.3/20)
3. autoFL (UV) (Zeiss Apo 6.3/20)
4. EPI (Nikon CFN PlanApo 10/0.45)
5. EPI (Zeiss Apo 6.3/20)
6. DIC (Nikon CFN Plan Fluor 40/0.75)
7. DIC (Nikon CFN Plan Fluor 40/0.75)

Lou Jost · Post by **Lou Jost** » Thu Jan 10, 2019 11:17 am

Thanks very much for the added information and for the second set! It is much appreciated.

ChrisR · Post by **ChrisR** » Thu Jan 10, 2019 11:35 am

Super pictures, again

I believe you are using afocal method, yes?
When you change from Zeiss to Nikon, do you change eyepieces?

I apologize if you have already covered this!

Jacek · Post by **Jacek** » Thu Jan 10, 2019 12:30 pm

Yes, I'm still doing it afokal, I do not change my glasses.
I have Chinese glasses 10X/20 with a rejected pupil.
I use Nikon CFWN 10X/20 glasses only for DIC.

pbraub · Post by **pbraub** » Thu Jan 10, 2019 12:40 pm

Spectacular images, thank you Jacek!

What lightsource do you use for UV excitation? Do you excite through the objective or from the side?

Kind regards
Peter

Robert Berdan · Post by **Robert Berdan** » Thu Jan 10, 2019 9:55 pm

Exceptional images Jacek you are an inspiration. Well done.
Rob

Sumguy01 · Post by **Sumguy01** » Thu Jan 10, 2019 11:49 pm

Excellent photos.
Thanks for sharing.

Jacek · Post by **Jacek** » Fri Jan 11, 2019 12:43 am

Thanks

I use fluorescence on two microscopes. On one FL epi (LED lighting 3W 365 nm (UV) and 460 nm (blue)).
On the second one I have FL dia, one diode 365 nm on it, a converted condenser, enlarging the diode + UG5 filter and barrier filter.
In addition, for small magnifications (10X lens and small) I use two illuminators that I have made (365 LEDs + UG5 excitation filters) - this solution is very efficient lighting.

Various

Various

Amazing Photomicrographs