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Living creatures under reflected light microscope?

 
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Jesse



Joined: 11 Dec 2016
Posts: 64

PostPosted: Thu Aug 16, 2018 8:51 am    Post subject: Living creatures under reflected light microscope? Reply with quote

Looking at microscopes, I see that Epi microscopes come with quite high magnifications.

Curiously, I don't think I've ever seen living creatures under a reflected light microscope. Why is that? What does pod life look like under a reflected light microscope?
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Alan Wood



Joined: 29 Dec 2010
Posts: 273
Location: Near London, U.K.

PostPosted: Thu Aug 16, 2018 9:53 am    Post subject: Reply with quote

Jesse

Depth of field is very small, you get a much better view with a stereomicroscope.

Dark-ground reflected light works fine for biological specimens, apart from the minimal depth of field.

Bright-field reflected light with biological specimens can give very poor results with lots of flare. Adjusting a polariser and analyser can help a bit.

Alan Wood
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Jesse



Joined: 11 Dec 2016
Posts: 64

PostPosted: Thu Aug 16, 2018 10:21 am    Post subject: Reply with quote

Alan Wood wrote:
Jesse

Depth of field is very small, you get a much better view with a stereomicroscope.

Dark-ground reflected light works fine for biological specimens, apart from the minimal depth of field.

Bright-field reflected light with biological specimens can give very poor results with lots of flare. Adjusting a polariser and analyser can help a bit.

Alan Wood


Can you provide some example photos? I'm just horribly curious.
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