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Spyrogyra nucleus

 
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Linden.g



Joined: 06 Apr 2009
Posts: 346
Location: PA USA

PostPosted: Sat Feb 24, 2018 11:04 am    Post subject: Spyrogyra nucleus Reply with quote

I found trying to image the nucleus very challenging as it's deep inside the cell and often masked by the chloroplasts. Focus stacked, 40x S-Plan APO DIC flash




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Linden Gledhill http://www.flickr.com/photos/13084997@N03/
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rjlittlefield
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Joined: 01 Aug 2006
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Location: Richland, Washington State, USA

PostPosted: Sat Feb 24, 2018 11:32 am    Post subject: Reply with quote

Very nice!

Does stereo rendering of the nucleus add anything good?

--Rik
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Linden.g



Joined: 06 Apr 2009
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Location: PA USA

PostPosted: Sat Feb 24, 2018 12:32 pm    Post subject: Reply with quote

Hi Rik, what would be the minimum number of steps i would need to render the stereo view
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Linden Gledhill http://www.flickr.com/photos/13084997@N03/
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Olympusman



Joined: 15 Jan 2012
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PostPosted: Sat Feb 24, 2018 2:51 pm    Post subject: Spirogyra Nucleus Reply with quote

Nice capture!

Mike
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Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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rjlittlefield
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PostPosted: Sat Feb 24, 2018 5:02 pm    Post subject: Reply with quote

Linden.g wrote:
what would be the minimum number of steps i would need to render the stereo view

There's no hard-and-fast number. I've seen good results from 20-ish frames. More important is the physical aspect ratio of the subject, depth:width. As that drops below 1:10 or so, the depth gets harder and harder to see.

Use the formulas at https://zerenesystems.com/cms/stacker/docs/syntheticstereo#calculating_shift_based_on_viewing_angle to calculate what shift % you need, based on your subject dimensions and viewing angles.

--Rik
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KurtM



Joined: 16 Jul 2014
Posts: 55
Location: League City, Texas

PostPosted: Sat Feb 24, 2018 6:58 pm    Post subject: Reply with quote

That's a fantastic image, Linden.g! Cool

Since Rik brings it up, how wonderful a 3-D look at it sounds. I love those Zerene synthetic cross-eye stereo images, really makes the this whole digital revolution worth the bother... Mr. Green
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Kurt Maurer
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Linden.g



Joined: 06 Apr 2009
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Location: PA USA

PostPosted: Sun Feb 25, 2018 7:47 am    Post subject: Reply with quote

Thanks for the additional information on viewing angle and step estimation Rik. I need to have another go at imaging the nucleus.
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