Rotifers, Tetraselmis, 1000 fps added vorticella at 2500 fps

Images made through a microscope. All subject types.

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Linden.g
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Rotifers, Tetraselmis, 1000 fps added vorticella at 2500 fps

Post by Linden.g »

I've been playing around with a high speed video camera. On loan from Hadland Imaging. The camera can do 10 million frames per second. http://hadlandimaging.com/shimadzu-hyper-vision-hpv-x2/ I've not used anywere near that frame rate yet, but have imaged rotifer and tetraselmis at 1000 fps.

Hi-speed video of rotifers at 500 fps (replayed at 24 fps). Taken using LED, DIC lighting with a 20x S-Plan Apo lens.

https://www.flickr.com/photos/13084997@ ... ed-public/

Hi-speed video of rotifers at 1000 fps (replayed at 24 fps). Taken using LED, DIC lighting with a 20x S-Plan Apo lens.

https://www.flickr.com/photos/13084997@ ... ed-public/

Hi-speed video of rotifers at 200 fps (both replayed at 24 fps). Taken using LED, DIC lighting with a 10x S-Plan Apo lens

https://www.flickr.com/photos/13084997@ ... ed-public/

This is a hi-speed video of Tetraselmis, a motile green algae. The video initially shows capture at 60 fps and then the second segment is at 1000 fps (both replayed at 24 fps). Taken using LED, DIC lighting with a 50x S-Plan Apo lens. It seems to take about 15 nanoseconds (actually 15 milliseconds, thanks to Rik for spotting the error) for a flagella to do one stroke.

https://www.flickr.com/photos/13084997@ ... ed-public/
Last edited by Linden.g on Sun Feb 18, 2018 6:00 pm, edited 3 times in total.

rjlittlefield
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Re: Video of Rotifers and Tetraselmis at 1000 fps

Post by rjlittlefield »

Linden.g wrote:It seems to take about 15 nanoseconds for a flagella to do one stroke.
Cool stuff!

But I think the labeling has led you astray. What I see in the video are time stamps like this:
Image
where it's the leading 69 that increments from one frame to another. But those two blocks of digits that I've outlined indicate that the 69 et.al. are actually millions of ns, that is, milliseconds.

So the way I see, it takes about 15 milliseconds to do one stroke. (That is, 15 frames at 1000 frames per second.)

--Rik

Linden.g
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Post by Linden.g »

Hi Rik, you are right I messed up my units. Thanks for noticing I'll correct it.

leonardturner
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Post by leonardturner »

What a great tool for studying rotifers "at work". Very interesting; thanks for posting!

Leonard

carlos.uruguay
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Post by carlos.uruguay »

Amazing!

micro_pix
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Post by micro_pix »

Great!

Can I borrow the camera when you are finished!

Linden.g
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Post by Linden.g »

Thanks for the kind comments.

Hi-speed video of a vorticella at 1000 fps and 2,500 fps for last segment (replayed at 24 fps). Taken using LED, DIC lighting with a 50x S-Plan Apo lens

https://www.flickr.com/photos/13084997@ ... ed-public/

rjlittlefield
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Post by rjlittlefield »

Very nice!

Just out of curiosity, what's a ballpark price for this little jewel?

--Rik

Linden.g
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Post by Linden.g »

Hi Rik, its list price is $250K :D I had to send it back but hope to experiment with this camera by the end of the week http://hadlandimaging.com/ix-cameras-i-speed-7-series/

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