www.photomacrography.net :: View topic - Rotifers, Tetraselmis, 1000 fps added vorticella at 2500 fps
www.photomacrography.net Forum Index
An online community dedicated to the practices of photomacrography, close-up and macro photography, and photomicrography.
Photomacrography Front Page Amateurmicrography Front Page
Old Forums/Galleries
 
 FAQFAQ   SearchSearch   MemberlistMemberlist   UsergroupsUsergroups   RegisterRegister 
 ProfileProfile   Log in to check your private messagesLog in to check your private messages   Log inLog in 
Rotifers, Tetraselmis, 1000 fps added vorticella at 2500 fps

 
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope
View previous topic :: View next topic  
Author Message
Linden.g



Joined: 06 Apr 2009
Posts: 346
Location: PA USA

PostPosted: Sun Feb 11, 2018 4:53 pm    Post subject: Rotifers, Tetraselmis, 1000 fps added vorticella at 2500 fps Reply with quote

I've been playing around with a high speed video camera. On loan from Hadland Imaging. The camera can do 10 million frames per second. http://hadlandimaging.com/shimadzu-hyper-vision-hpv-x2/ I've not used anywere near that frame rate yet, but have imaged rotifer and tetraselmis at 1000 fps.

Hi-speed video of rotifers at 500 fps (replayed at 24 fps). Taken using LED, DIC lighting with a 20x S-Plan Apo lens.

https://www.flickr.com/photos/13084997@N03/26340285108/in/dateposted-public/

Hi-speed video of rotifers at 1000 fps (replayed at 24 fps). Taken using LED, DIC lighting with a 20x S-Plan Apo lens.

https://www.flickr.com/photos/13084997@N03/28433235989/in/dateposted-public/

Hi-speed video of rotifers at 200 fps (both replayed at 24 fps). Taken using LED, DIC lighting with a 10x S-Plan Apo lens

https://www.flickr.com/photos/13084997@N03/28433182579/in/dateposted-public/

This is a hi-speed video of Tetraselmis, a motile green algae. The video initially shows capture at 60 fps and then the second segment is at 1000 fps (both replayed at 24 fps). Taken using LED, DIC lighting with a 50x S-Plan Apo lens. It seems to take about 15 nanoseconds (actually 15 milliseconds, thanks to Rik for spotting the error) for a flagella to do one stroke.

https://www.flickr.com/photos/13084997@N03/25341554317/in/dateposted-public/
_________________
Linden Gledhill http://www.flickr.com/photos/13084997@N03/


Last edited by Linden.g on Sun Feb 18, 2018 6:00 pm; edited 3 times in total
Back to top
View user's profile Send private message Send e-mail Visit poster's website
rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 19393
Location: Richland, Washington State, USA

PostPosted: Sun Feb 11, 2018 6:22 pm    Post subject: Re: Video of Rotifers and Tetraselmis at 1000 fps Reply with quote

Linden.g wrote:
It seems to take about 15 nanoseconds for a flagella to do one stroke.

Cool stuff!

But I think the labeling has led you astray. What I see in the video are time stamps like this:

where it's the leading 69 that increments from one frame to another. But those two blocks of digits that I've outlined indicate that the 69 et.al. are actually millions of ns, that is, milliseconds.

So the way I see, it takes about 15 milliseconds to do one stroke. (That is, 15 frames at 1000 frames per second.)

--Rik
Back to top
View user's profile Send private message Visit poster's website
Linden.g



Joined: 06 Apr 2009
Posts: 346
Location: PA USA

PostPosted: Sun Feb 11, 2018 6:51 pm    Post subject: Reply with quote

Hi Rik, you are right I messed up my units. Thanks for noticing I'll correct it.
_________________
Linden Gledhill http://www.flickr.com/photos/13084997@N03/
Back to top
View user's profile Send private message Send e-mail Visit poster's website
leonardturner



Joined: 14 Mar 2013
Posts: 431
Location: Atlanta, GA, USA

PostPosted: Wed Feb 14, 2018 12:15 pm    Post subject: Reply with quote

What a great tool for studying rotifers "at work". Very interesting; thanks for posting!

Leonard
Back to top
View user's profile Send private message
carlos.uruguay



Joined: 23 Feb 2012
Posts: 5053
Location: Uruguay - Montevideo - America del Sur

PostPosted: Fri Feb 16, 2018 5:30 am    Post subject: Reply with quote

Amazing!
Back to top
View user's profile Send private message Send e-mail Visit poster's website
micro_pix



Joined: 11 May 2012
Posts: 141

PostPosted: Sat Feb 17, 2018 4:00 am    Post subject: Reply with quote

Great!

Can I borrow the camera when you are finished!
Back to top
View user's profile Send private message
Linden.g



Joined: 06 Apr 2009
Posts: 346
Location: PA USA

PostPosted: Sun Feb 18, 2018 5:56 pm    Post subject: Reply with quote

Thanks for the kind comments.

Hi-speed video of a vorticella at 1000 fps and 2,500 fps for last segment (replayed at 24 fps). Taken using LED, DIC lighting with a 50x S-Plan Apo lens

https://www.flickr.com/photos/13084997@N03/39637388664/in/dateposted-public/
_________________
Linden Gledhill http://www.flickr.com/photos/13084997@N03/
Back to top
View user's profile Send private message Send e-mail Visit poster's website
rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 19393
Location: Richland, Washington State, USA

PostPosted: Sun Feb 18, 2018 7:49 pm    Post subject: Reply with quote

Very nice!

Just out of curiosity, what's a ballpark price for this little jewel?

--Rik
Back to top
View user's profile Send private message Visit poster's website
Linden.g



Joined: 06 Apr 2009
Posts: 346
Location: PA USA

PostPosted: Sun Feb 18, 2018 9:09 pm    Post subject: Reply with quote

Hi Rik, its list price is $250K Very Happy I had to send it back but hope to experiment with this camera by the end of the week http://hadlandimaging.com/ix-cameras-i-speed-7-series/
_________________
Linden Gledhill http://www.flickr.com/photos/13084997@N03/
Back to top
View user's profile Send private message Send e-mail Visit poster's website
Display posts from previous:   
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope All times are GMT - 7 Hours
Page 1 of 1

 
Jump to:  
You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot vote in polls in this forum


Powered by phpBB © 2001, 2005 phpBB Group