www.photomacrography.net :: View topic - Experiments with Russian fluorescence > Update >
www.photomacrography.net Forum Index
An online community dedicated to the practices of photomacrography, close-up and macro photography, and photomicrography.
Photomacrography Front Page Amateurmicrography Front Page
Old Forums/Galleries
 
 FAQFAQ   SearchSearch   MemberlistMemberlist   UsergroupsUsergroups   RegisterRegister 
 ProfileProfile   Log in to check your private messagesLog in to check your private messages   Log inLog in 
Experiments with Russian fluorescence > Update >

 
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope
View previous topic :: View next topic  
Author Message
WalterD



Joined: 06 Jul 2015
Posts: 361
Location: Rotterdam, the Netherlands

PostPosted: Sun Feb 11, 2018 5:58 am    Post subject: Experiments with Russian fluorescence > Update > Reply with quote

My current project is "retrofitting" a russian Lomo Lumam R1 fluorescence microscope. (you can read about that process here: https://www.photomacrography.net/forum/viewtopic.php?t=36484 )
This was the scope I've been using when I started to post in this forum about three years ago, without fluorescence though.

Observed some stuff that was found readily available: pollen (unmounted)(1), the tip of a geranium leaf (2)and moss(3&4).
Objectives used 40x/0.65 (1) , the rest the lowest magnification 9x/0.20. This is epi-fluorescence; the light is coming from above and directed through the objective.
For pollen the green filter block was used, the other 3 were done with the blue block. The geranium leaf was green for real; due to fluorescence the chlorophyl shows up red in the picture.
Somehow with the moss (that also contains chlorophyl) that turned out different, though for both the same exciter filter was utilized.
An interesting aspect of fluorescence is that one can stain without actually staining, besides the red chlorophyl, noted cellulose like material turns up white.








Thanks for looking!
_________________
www.waltermachielsen.com


Last edited by WalterD on Sun Feb 18, 2018 12:07 am; edited 1 time in total
Back to top
View user's profile Send private message Visit poster's website
micro_pix



Joined: 11 May 2012
Posts: 141

PostPosted: Sun Feb 11, 2018 12:18 pm    Post subject: Reply with quote

Good work. I think the moss images are very nice.
Back to top
View user's profile Send private message
Jacek



Joined: 02 Oct 2011
Posts: 5030
Location: Poland

PostPosted: Mon Feb 12, 2018 12:24 am    Post subject: Reply with quote

Good job !
_________________
https://photos.app.goo.gl/MV9IynUtBdaKkVj53
Back to top
View user's profile Send private message Send e-mail
sushidelic



Joined: 10 Jun 2016
Posts: 205

PostPosted: Mon Feb 12, 2018 7:32 am    Post subject: Reply with quote

Moss looks great!
Back to top
View user's profile Send private message
carlos.uruguay



Joined: 23 Feb 2012
Posts: 4972
Location: Uruguay - Montevideo - America del Sur

PostPosted: Thu Feb 15, 2018 5:10 am    Post subject: Reply with quote

Super!
Back to top
View user's profile Send private message Send e-mail Visit poster's website
WalterD



Joined: 06 Jul 2015
Posts: 361
Location: Rotterdam, the Netherlands

PostPosted: Sun Feb 18, 2018 12:11 am    Post subject: Reply with quote

Good to hear that. You mentioned moss in particular, I agree that moss can be a very good match with the blue filter block I've been using.
So continued with the same sample, that was actually taken about 5 months ago and kept a bit moist since then. The next three images may give a better impression of the process; it almost looks like parts of the moss have turned into a lamp!

The light from the HBO100 mercury arc lamp does have an impact on the material, sometimes it looks like the vulnerable moss leafs are shriveling up.
Furthermore the (a.o. red) radiation from the leafs is initially the strongest and weakens within minutes, e.g. in photo 6 the session started with red radiation and halfway that slowly turned into green. For this image the combination of shriveling and colour change made the actual stacking more difficult (or "challenging" I should say). The upper/right half of that picture was done last, (second half of the stack) you can see the colour difference.
In picture 7 there is a significant difference in brightness between the left and right part. Basically this is how the fluorescence worked out on that piece of moss, while the settings remained identical.
The many different colours in picture 5 were actually there, (not enhanced whatsoever) but became more distinct when doing the stack.

For these sessions a medium frame Canon 70D was utilized, like all my other pictures. In this case without the afocal setup, so the image coming from the objective projected straightaway on the sensor.
Keep an eye on further updates (also of my post in equipment discussions) as I fortunately was able to find a very reasonably priced full frame body!

Note: all 9x/0.21 epi objective.




_________________
www.waltermachielsen.com
Back to top
View user's profile Send private message Visit poster's website
carlos.uruguay



Joined: 23 Feb 2012
Posts: 4972
Location: Uruguay - Montevideo - America del Sur

PostPosted: Sun Feb 18, 2018 5:40 am    Post subject: Reply with quote

Back to top
View user's profile Send private message Send e-mail Visit poster's website
GaryB



Joined: 29 Jul 2016
Posts: 521

PostPosted: Mon Feb 19, 2018 5:28 pm    Post subject: Reply with quote

Those are some spectacular colors, very well done.
Back to top
View user's profile Send private message
Display posts from previous:   
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope All times are GMT - 7 Hours
Page 1 of 1

 
Jump to:  
You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot vote in polls in this forum


Powered by phpBB © 2001, 2005 phpBB Group