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Fungal Spores

 
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micro_pix



Joined: 11 May 2012
Posts: 141

PostPosted: Fri Feb 02, 2018 4:15 pm    Post subject: Fungal Spores Reply with quote

Here are some photos of fungal spores and the basidiocarps that produced them.

They are all taken under the 100X oil objective (with the exception of the Entoloma which was 40x). They vary in size but are between 7 and 18 microns in length. The first four are from the same genus.
The spores are as photographed, the background has been cleaned and I thought they looked better with a slight shadow which I added in Photoshop.

Panaeolus semiovatus




Panaeolus acuminatus



Panaeolus fimicola



Panaeolus papilionaceus



Hypholoma ericaeum



Inocybe stellatospora



Last edited by micro_pix on Mon Feb 05, 2018 10:56 am; edited 2 times in total
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Chris S.
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Joined: 05 Apr 2009
Posts: 3071
Location: Ohio, USA

PostPosted: Fri Feb 02, 2018 10:47 pm    Post subject: Reply with quote

Wonderful! Very effective to see field shots of the fruiting bodies juxtaposed with the spores as you have done. And to have four species of the Panaeolus genus placed so neatly for comparison. Very Happy

Did you treat any of those spores with KOH?

--Chris S.
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micro_pix



Joined: 11 May 2012
Posts: 141

PostPosted: Sat Feb 03, 2018 2:12 am    Post subject: Reply with quote

Thanks Chris. No I didn’t use KOH with these, they were in water. The Panaeolus spores are all naturally dark, the spore print is what I’d calll black/brown, and the Hypholoma and Inocybe spores are mid brown.

I always try and avoid using KOH on fresh spores as I get a better record of their natural colour through the scope but you do need good resolution (or DIC) to see the hyaline spores easily. I have often used lacto-phenol cotton blue if I’m looking for surface ornamentation on the spores but if you have it, DIC is great for that too.
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Pitufo



Joined: 21 Jun 2015
Posts: 206
Location: United Kingdom

PostPosted: Sat Feb 03, 2018 3:36 am    Post subject: Reply with quote

Very nice work and good to have photographs of the spores. This group can be hard to tell apart and spore descriptions are not nearly as helpful.

I didn't realise that KOH had an effect on the colour of spores but it can change their size slightly I believe.
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micro_pix



Joined: 11 May 2012
Posts: 141

PostPosted: Sat Feb 03, 2018 4:13 am    Post subject: Reply with quote

Thanks Pitufo. It’s a long time since I looked at spores in KOH as I invariably look at fresh spores in water. I thought I remember that it made the spores appear a bit darker/browner but it isn’t regarded as a stain.
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leonardturner



Joined: 14 Mar 2013
Posts: 431
Location: Atlanta, GA, USA

PostPosted: Sat Feb 03, 2018 4:18 am    Post subject: Reply with quote

Very interesting comparisons, nicely done and presented. The discussion of KOH is interesting as well. As you may know, the "KOH prep" has often been used diagnostically in medicine to dissolve away flakes of skin gently scraped from a rash, revealing the fungal elements within. I have often done this to good effect.
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micro_pix



Joined: 11 May 2012
Posts: 141

PostPosted: Sat Feb 03, 2018 7:03 am    Post subject: Reply with quote

Thanks Leonard. I knew KOH was caustic and damaging to skin but I hadn't realised it was used for that purpose.

Here are three photos of hyaline (clear/white) spores. All in water; the first is in brightfield, the second is in darkfield and the third in DIC.

Helvella crispa spores in water.



Entoloma conferendum spores in darkfield.



Laccaria laccata spores in DIC.

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Pitufo



Joined: 21 Jun 2015
Posts: 206
Location: United Kingdom

PostPosted: Sat Feb 03, 2018 9:39 am    Post subject: Reply with quote

Great images Smile

KMn04 3% can also be used to darken spore colour and allows hyaline spores to be seen more easily https://micro-science.co.uk/product/potassium-permanganate/
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Sumguy01



Joined: 28 Jan 2013
Posts: 1196
Location: Ketchikan Alaska USA

PostPosted: Sat Feb 03, 2018 9:47 am    Post subject: Reply with quote

Very nice.
Interesting and informative.
Thanks for sharing.
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Jacek



Joined: 02 Oct 2011
Posts: 5123
Location: Poland

PostPosted: Sun Feb 04, 2018 3:29 am    Post subject: Reply with quote

Good job, very nice
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vasselle



Joined: 05 Jan 2014
Posts: 1497
Location: France

PostPosted: Sun Feb 04, 2018 2:40 pm    Post subject: Reply with quote

très bon travail et les images sont belles
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micro_pix



Joined: 11 May 2012
Posts: 141

PostPosted: Sun Feb 04, 2018 2:47 pm    Post subject: Reply with quote

Thanks for the nice comments.

Dave
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Walter Piorkowski



Joined: 14 Aug 2006
Posts: 684
Location: South Beloit, Ill

PostPosted: Sun Feb 04, 2018 9:53 pm    Post subject: Reply with quote

Very nice project. Enjoying your images. Walt
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ChrisR
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Joined: 14 Mar 2009
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Location: Near London, UK

PostPosted: Mon Feb 05, 2018 5:34 am    Post subject: Reply with quote

+1 enjoying these. What size are the spores, approx?
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Chris R
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micro_pix



Joined: 11 May 2012
Posts: 141

PostPosted: Mon Feb 05, 2018 10:47 am    Post subject: Reply with quote

Thanks for the comments.

Hi Chris,
The spores are roughly around 8 or 9 microns for the Inocybe spore (they are not all depicted at the same scale) to around 18 microns for the largest of the Panaeolus spores (Panaeolus semiovatus). The Entoloma darkfield was a 40X objective rather than the 100X.

Dave
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