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Amoeba nucleus

 
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Olympusman



Joined: 15 Jan 2012
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PostPosted: Sun Jan 28, 2018 9:45 am    Post subject: Amoeba nucleus Reply with quote

Amoeba nucleus



My first attempt at 3-D


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Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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Olympusman



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PostPosted: Sun Jan 28, 2018 9:50 am    Post subject: Amoeba nucleus Reply with quote

Resized images - another try -


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Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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rjlittlefield
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Joined: 01 Aug 2006
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Location: Richland, Washington State, USA

PostPosted: Sun Jan 28, 2018 11:10 am    Post subject: Reply with quote

This is not working in 3D. Most of the details are not matchable between the two frames, certainly due to difference in focus and apparently also due to physical movement within the body.

What method did you use to acquire these two images?

--Rik
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Olympusman



Joined: 15 Jan 2012
Posts: 3796

PostPosted: Mon Jan 29, 2018 9:05 am    Post subject: Nucleus Reply with quote

The amoeba was dead, so there was no movement. It was killed with glutaraldehyde, which causes amoebae to constrict.
It was shot with a 60X objective. Both sets were focus stacked. The shift between the focus stack sets is hard to quantify - maybe 10%.

Mike
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Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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