Trichomes at 20x and 80x

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

concon
Posts: 87
Joined: Thu Jun 01, 2017 6:21 am

Trichomes at 20x and 80x

Post by concon »

First time sharing here and would definitely appreciate any constructive criticism- always looking to optimize and improve. Over-sharpened for IG and backgrounds replaced.

I'll update from the top down :)



Mitutoyo BD Plan 80x + Vivitar 200mm f/3.5 @ 170 images
Image

Mitutoyo BD Plan 20x + Vivitar 200mm f/3.5 @ 204 images
Image

Mitutoyo BD Plan 80x + Vivitar 200mm f/3.5 @ 508 images
Image

Mitutoyo BD Plan 20x + Raynox DCR-150 @ 239 images
Image

Mitutoyo BD Plan 80x + Raynox DCR-150 @ 161 images
Image
Last edited by concon on Fri Jan 26, 2018 1:27 pm, edited 2 times in total.

Marek Mis
Posts: 2586
Joined: Sat Jul 10, 2010 9:56 am
Location: Suwalki, Poland
Contact:

Post by Marek Mis »

Nice images ! Welcome :)

Marek

grgh
Posts: 372
Joined: Sat Mar 09, 2013 4:55 am
Location: Lancashire. UK

Post by grgh »

Very nice images.
envious of the objectives.

George
used to do astronomy.
and photography.
Zeiss Universal Phase contrast.
Zeiss PMII
B&L stereo zoom.

Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Post by Charles Krebs »

Very graphic and interesting. Welcome, and keep putting up images!

Jacek
Posts: 5357
Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

Very nice

concon
Posts: 87
Joined: Thu Jun 01, 2017 6:21 am

Post by concon »

Thanks for the compliments and welcomes :) I'll definitely share more as I'm looking to improve everyday! I'll probably just append to this thread as not to create redundancy

carlos.uruguay
Posts: 5358
Joined: Thu Feb 23, 2012 8:05 pm
Location: Uruguay - Montevideo - America del Sur
Contact:

Post by carlos.uruguay »

Nice!!!! Welcome!

concon
Posts: 87
Joined: Thu Jun 01, 2017 6:21 am

Post by concon »

Updated :D

rjlittlefield
Site Admin
Posts: 23562
Joined: Tue Aug 01, 2006 8:34 am
Location: Richland, Washington State, USA
Contact:

Post by rjlittlefield »

These all look good, once I got past the funny face watermark.

What was the nature of the update?

--Rik

concon
Posts: 87
Joined: Thu Jun 01, 2017 6:21 am

Post by concon »

Haha, sorry; these were just ready go. The people in the cannabis industry aren't very versed(or care) regarding copyright so they take images left and right. I plaster a logo right in the middle as a small layer of protection.

I have another post somewhere and another member stated interest in seeing more. :)

Chris S.
Site Admin
Posts: 4042
Joined: Sun Apr 05, 2009 9:55 pm
Location: Ohio, USA

Post by Chris S. »

Concon, these are interesting and well executed. Thanks for posting! :D

Must admit that I find "updating" somewhat disorienting, as I can't compare "current" with "before." And admit to finding the watermarks a bit disconcerting--though I sympathize with your desire to avoid image theft.

What is the red juice in the trichomes shown in your fourth image down? Does it start out filling the shaft of the trichome, then recede as the sample dries out?

Can you discuss the color differences--clear, greenish, golden--are these representative of differences between cultivars, attributable to the lighting, or something else?

Cheers,

--Chris S.

concon
Posts: 87
Joined: Thu Jun 01, 2017 6:21 am

Post by concon »

Thanks :)

The red in the stalks are anthocyanins. They're pigments that can be a variety of colors, but they're mainly red in cannabis due to pH control. They can appear in the leaf cells or certain(capitate-stalked) trichome stalks- very rarely they'll push up to the cells that connect the head to the stalk (basal and stipe cells).

The cannabis community DIES for reds/purples- it's a desired aesthetic trait. The presence and location isn't affected by dryness as much as a variety of other variables that haven't been isolated. It's mostly dependent on genetics as some strains will never exhibit any reds or purples.

Until recently, I've had a hard time getting my hands on samples to shoot and pursue personal research. There were a few things I wanted to look into with all my might- the differences in head color/activity compared to cannabinoid contents. Cannabinoids are the most desired molecules and are primarily in the heads. They'll go through various transformations throughout a plant's life. Growers have referred to head color for ages as the measuring stick for when a plant is ready to be harvested- 'wait until it's amber'.

Through my personal observation I've documented that cloudy/bubbly heads are most prevalent during the earlier weeks of the flowering stage (I'll now be able to see which cannabinoids are most present at this time with the help of the lab). Ambering occurs towards the end of the flowering cycle, which is dependent on strain/genetics.

An interesting thing regarding the ambering is that it actually occurs at the base of the head and top of the stalk, at the basal and stipe cells. The color is then reflected into the head and to the naked eye it appears as if the whole head is amber. Depending on viewing angle an older trichome can appear clear or amber. This is a semi-recent discovery made by my mentor that we've been able document many times again.

Most of the trichome is transparent, mind the shadows caused by depth changes, various vacuolar molecules, whatever is behind it, and the base of it. I think most of greening I capture in the stalks is due to depth differences or shooting against the leaf.

----Edit----
A few months ago I was stepping through a stack and noticed a bit of cytoplasmic streaming. I've since seen this in a good amount of my higher magnification stacks. I retouch as needed, but thought it was worth sharing as it was fairly interesting; I'm looking into a way of getting enough light to capture a video.



Thanks for the questions :D

Post Reply Previous topicNext topic