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10x microscope objective kit for hobby

 
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anvancy



Joined: 05 Dec 2009
Posts: 351
Location: India

PostPosted: Fri Dec 22, 2017 2:00 am    Post subject: 10x microscope objective kit for hobby Reply with quote

Hi All,

I have been going in detail in the FAQ section for the microscope objectives and have got the general idea of the workings.

I am a hobbyist photographer from India so regular access to precise optics and kits is difficult. Yes ebay/aliexpress are options but its a big luck with customs.

Before I begin my question here is my equipment list.

Canon 80D
100mm,55-250mm and MPE 65mm.
Raynox 150 250 and MSN 202.
Wemacro Rail.
Ikea Jansos.

Since i do only hobby, I am looking to build a decent objective rig that can run on my existing setup and is not heavy on the pocket. Given that I have Raynox 150 and 250 and the 55-250 which can be used as tube lenses, the best option looks like the infinity objectives.

The MPE can handle good magnification till 5x. So post 5x to 10x is my lookout.

I am still unable to understand how the raynox 150 is mounted to the camera? is it on the extension tube and then performs as a tube lens?

Things can be brought down from the US personally so carriage is not much of a problem.

I checked Edmond Optics website but the 10x infinity objectives runs in thousand of dollars. If I am correct Robert has tested the wemacro's 10x objective too?

I am building a list of what all to buy to do this but unable to pinpoint the accessories.

Any help/links will be appreciated.

Thanks

Anvancy
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Raynox 150|Raynox 250|Raynox MSN 202|Canon MPE 65mm|Canon 100mm.|Wemacro Rail
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glmory



Joined: 30 Dec 2016
Posts: 25

PostPosted: Fri Dec 22, 2017 3:02 am    Post subject: My system Reply with quote

I put together a labeled photo in the review for this objective on Amazon. The photo and description may help:

https://www.amazon.com/gp/customer-reviews/RZ2ZHD61HTR4/

The Mitutoyo 5X and 10X are both good choices for what you want to do. As is this Nikon 10X objective:
https://www.amazon.com/CFI-10x-Microscope-Objective/dp/B01AOCERHA/
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anvancy



Joined: 05 Dec 2009
Posts: 351
Location: India

PostPosted: Mon Dec 25, 2017 12:08 am    Post subject: Reply with quote

Thank you. That really helps.

Though given my situation, finances and budget kept in mind, the famous objectives are beyond the budget. $600 is way off for me given that I am not even earning from my photos.

The ones from wemacro do seem within the budget.
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Raynox 150|Raynox 250|Raynox MSN 202|Canon MPE 65mm|Canon 100mm.|Wemacro Rail
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Adalbert



Joined: 30 Nov 2015
Posts: 363

PostPosted: Mon Dec 25, 2017 4:31 am    Post subject: Reply with quote

Hello Anvancy,
What about the MRN70100:
https://microscopecentral.com/products/nikon-eclipse-e100-microscope-objectives
http://www.photomacrography.net/forum/viewtopic.php?t=30241&highlight=
BR, ADi
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 18908
Location: Richland, Washington State, USA

PostPosted: Mon Dec 25, 2017 11:58 am    Post subject: Reply with quote

Adalbert wrote:
What about the MRN70100

Good suggestion! I agree that the MRN7100 is an excellent low-cost objective. On APS-C, it gives good coverage even to the corners when pushed down to 5X using a 100 mm tube lens. See for example http://www.photomacrography.net/forum/viewtopic.php?t=16348

Quote:
https://microscopecentral.com/products/nikon-eclipse-e100-microscope-objectives

Thanks for the link to Microscope Central. I was not aware that the MRN70100 could be purchased from anybody except Nikon these days.

anvancy wrote:
I am still unable to understand how the raynox 150 is mounted to the camera? is it on the extension tube and then performs as a tube lens?

Yes. See http://www.photomacrography.net/forum/viewtopic.php?t=35350 for illustration and discussion.

--Rik
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Adalbert



Joined: 30 Nov 2015
Posts: 363

PostPosted: Mon Dec 25, 2017 1:13 pm    Post subject: Reply with quote

Hello Rik,
In the following thread you show a construction with a really small distance between the tube-lens and the microscope-objective.
http://www.photomacrography.net/forum/viewtopic.php?t=35350

According to the recommendation of NIKON the distance should be between 100mm and 200mm.
https://www.edmundoptics.com/microscopy/infinity-corrected-objectives/nikon-cfi60-infinity-corrected-brightfield-objectives/
Does this distance depend on the tube-lens?

If I used the Sigma LSA as a tube-lens, would I be able to reduce this distance to e.g. 50mm?

BR, ADi
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anvancy



Joined: 05 Dec 2009
Posts: 351
Location: India

PostPosted: Wed Dec 27, 2017 10:30 pm    Post subject: Reply with quote

Thanks Adalbert. This is a good suggestion and I am looking into it.

So these look like infinity objectives and can go on my 55-250mm as tube giving me the advantage to adjust my magnification.

A question though, whats the difference between E100 objectives and E200 objectives?
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 18908
Location: Richland, Washington State, USA

PostPosted: Wed Dec 27, 2017 11:04 pm    Post subject: Reply with quote

Adalbert wrote:
According to the recommendation of NIKON the distance should be between 100mm and 200mm.
https://www.edmundoptics.com/microscopy/infinity-corrected-objectives/nikon-cfi60-infinity-corrected-brightfield-objectives/
Does this distance depend on the tube-lens?

Yes, it depends on the tube lens. Beyond a certain distance you'll get vignetting, depending on the sensor size and the tube lens diameter. At very close distances you may or may not get slight degradation due to aberrations. To be honest I suspect that the specification of 100mm minimum distance is pretty arbitrary even for Nikon's tube lens.

Quote:
If I used the Sigma LSA as a tube-lens, would I be able to reduce this distance to e.g. 50mm?

I have never tested with that lens so I don't know for sure. But yes, that's what I would expect.

--Rik
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 18908
Location: Richland, Washington State, USA

PostPosted: Wed Dec 27, 2017 11:10 pm    Post subject: Reply with quote

anvancy wrote:
whats the difference between E100 objectives and E200 objectives?

According to https://www.nikoninstruments.com/Products/Upright-Microscopes/Educational/Eclipse-E100-LED/Model-Comparison, the E100 uses CFI45 objectives while the E200 uses CFI60.

That will be a matter of thread size (RMS for CFI45 versus 25mm for CFI60) and parfocal distance (from mounting threads to focused subject, 45mm versus 60 mm).

In terms of mounting in front of tube lens on a camera, it's just a matter of the adapter thread and how long the objective is.

--Rik
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anvancy



Joined: 05 Dec 2009
Posts: 351
Location: India

PostPosted: Tue Jan 02, 2018 8:48 pm    Post subject: Reply with quote

Thanks Rik.

Given that it will be my first foray into the microscope objective world, going slow on the budget looks logical.
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Raynox 150|Raynox 250|Raynox MSN 202|Canon MPE 65mm|Canon 100mm.|Wemacro Rail
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anvancy



Joined: 05 Dec 2009
Posts: 351
Location: India

PostPosted: Tue Jan 16, 2018 10:18 pm    Post subject: Reply with quote

Ok so update.

My sister has given me a deadline of 31 Jan for the parts to reach at Bellevue/Seattle WA. Which means I have to source my items in the US.

Taking Adalbert's suggestion, the nikon objectives can be shipped within so I dont think that is an issue.

If I am using the 55-250 or the canon 100mm as my tube lens, I will be requiring the adapters to attach the objectives. Any leads on that?

Extension tubes can be bought in India so thats not an issue. But any intermediary adapters are hard to get in India.

Can anybody help me out in preparing a shopping list preferably items that can be sourced in the US? Still little confused on the items that need to be sourced.

Thanks

Anvay
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Raynox 150|Raynox 250|Raynox MSN 202|Canon MPE 65mm|Canon 100mm.|Wemacro Rail
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Adalbert



Joined: 30 Nov 2015
Posts: 363

PostPosted: Wed Jan 17, 2018 2:55 am    Post subject: Reply with quote

Hi Anvay,

Please take a look at that:

https://www.ebay.com/itm/RISE-UK-58-42MM-58MM-42MM-58-to-42-Step-Down-Ring-Filter-Adapter/262339312015?hash=item3d14a4398f:g:6usAAOSw0JpV6Vzv
It has probably 42x0,75 but should match to the x1 :-)

https://www.ebay.com/itm/Camera-Adapter-For-RMS-Royal-Microscopy-Society-Lens-to-M42-Mount-Adapter/182621819125?hash=item2a851bd0f5:g:jjEAAOSwMvtZQjG1


Alternative with M42x1 but via 52mm:
https://www.ebay.com/itm/RISE-UK-58-52MM-58-MM-52-MM-58-to-52-Step-Down-Ring-Filter-Adapter/302032283738?hash=item465286e85a:g:W7kAAOSwA3tXou1p
https://www.ebay.com/itm/52mm-42mm-52-42-Step-Down-Filter-Ring-Stepping-Adapter/231123517331?ssPageName=STRK%3AMEBIDX%3AIT&_trksid=p2057872.m2749.l2649

BTW, you can ask for the MRN70100 in the local subsidiary of NIKON in India

BR, ADi
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zzffnn



Joined: 22 May 2014
Posts: 1670
Location: Texas USA

PostPosted: Wed Jan 17, 2018 7:24 am    Post subject: Reply with quote

anvancy wrote:
If I am using the 55-250 or the canon 100mm as my tube lens, I will be requiring the adapters to attach the objectives. Any leads on that?


Anvay,

Your Canon 100mm prime would likely vignette on APSC sensor (edit: please disregard that, since Rik uses Canon APSC and already said the 100mm prime works that way), likewise if you use the 55-250 zoom and go shorter than 200mm (but it should work at 250mm). A Canon macrophotographer can tell you for sure.
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