Hippeastrum flower pollen

Images made through a microscope. All subject types.

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JH
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Hippeastrum flower pollen

Post by JH »

Hippeastrum flower pollen
Nikon ELWD 100x, Canon 6D, EPI-light, Zerene Stacker Adobe PS and LR, Topaz ReMask 5. I used quite a lot of sharpening, and blurred out the background.

Best regards
Jörgen Hellberg

Image

Image
Jörgen Hellberg, my webbsite www.hellberg.photo

rjlittlefield
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Post by rjlittlefield »

Impressive! How deep is the stack (frames & microns) ?

--Rik

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Post by Pau »

Excellent, the stereo helps a lot to see the actual shape of the pollen texture
Pau

JH
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Post by JH »

Thanks Rik and Pau!
rjlittlefield wrote:Impressive! How deep is the stack (frames & microns) ?

--Rik
The lens is NA 0.8 so usually a step size close to 0.0005 mm (0.5 microns) will be OK with a little marginal.

I was trying to do a depth map where the dents could be seen. To do this I had to have smaller than normal steps. In this case I used 0.0001 mm (0.1 micron), so 319 pictures covered the distance of approx. 0.032 mm (32 microns).


Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

Jacek
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Post by Jacek »

Good job, Very nice

Ken Ramos
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Post by Ken Ramos »

So alien, so unearthly in appearance. Wonderful photograph!

JH
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Post by JH »

Thanks Ken Ramos and Jacek!
Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

carlos.uruguay
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Post by carlos.uruguay »

Nice!

Olympusman
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Pollen

Post by Olympusman »

Lovely.

Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

JH
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Post by JH »

Thanks Carlos and Mike.

Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

JH
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Post by JH »

I was interested in trying to have a clean black background. Tried some different options. For this BW-picture I used a Nikon plan apo 40x BD 210 mm lens, dark field, and green light (GIF-filter). The green light helped even though this was an APO-lens.


Image

And a 2D stereo made with Zerene.

Image

Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

Saul
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Post by Saul »

Jörgen , result is really beautiful !
Saul
μ-stuff

JH
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Post by JH »

Thanks Saul :D

Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

WalterD
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Post by WalterD »

Congratulations, the green light filter turns out to be a very good choice.
:)

Best regards,

Walter

Lou Jost
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Post by Lou Jost »

Beautiful monochrome shot. If you want to push this farther, you might want to use violet lasers and a cooled monochrome camera.....you'd achieve 20% more resolution and get smooth backgrounds with no thermal noise.

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