Bovine Pulmonary Artery Nucleus

Choronzon · Post by **Choronzon** » Wed Nov 15, 2017 1:48 pm

Usually when these nuclei are fixed, the genetic material is destroyed, but here we see some of it having been ejected in the fixation process and preserved.

Nikon N SIM 405 laser excitation 3D reconstruction
Nikon Super Resolution Apo TIRF 1.49NA

Pau · Post by **Pau** » Thu Nov 16, 2017 4:04 am

Is it one of the triple stained slides made or distributed by Michael Davidson's lab? I have a pair and of course I can't get close to this super-resolution

Choronzon · Post by **Choronzon** » Thu Nov 16, 2017 7:28 am

Yes, it's one of those triple stain slides that cost a fortune. I go through a lot of them as they fade quickly with intense excitation. I just left out the other 2 wavelengths so you can see the nuclear material easily.
They're great test slides because they're so thin and any non uniformity of emission shows up dramatically

carlos.uruguay · Post by **carlos.uruguay** » Thu Nov 16, 2017 4:44 pm