Fern sporangium in fluorescence light

Images made through a microscope. All subject types.

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Pau
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Post by Pau »

Lou Jost wrote:Do you think something like this could be done without filters by just killing the blue channel and reducing the green channel?
You could test it yourself, but I don't think so for two reasons that when combined could make inviable your suggested approach:
- Sensor photosites (actually their filters) are, in some some extent like your retina receptors, wideband sensitive
- fluorescence emission is MUCH weaker than excitation light.
Pau

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