Simocephalus Vetulus in Fluo and Pol

[hkv]

I found a rather big Simocephalus Vetulus in a recently collected water sample. She turned out to have small females under her carapace. In females, there is a large space between the back and the dorsal side of the carapace that functions as a brood pouch. I can spot at least 10 small females. Simocephalus Vetulus reproduces parthenogenetically most of the year (producing females).

Polarized light with 4X objective, size of the animal is just below 3 mm:




Fluorescent light with 4X objective:



Close-up with the 10X objective. Fluorescent light. Showing the small females (which were moving around)



The last image is a smaller female, just below 1 mm. In "poor man's" darkfield. I did not use a proper darkfield stop because I did not have one mounted in the condenser turret, but I instead set the condenser between two DIC positions having the metal frames of the DIC prisms act as a darkfield stop. Not very sophisticated, but I was somewhat surprised how well it worked.

[CEN]

Superb images!

I like the "poor man's" darkfield image most. Although I got a darkfield stop I often use the postion between two phase contrast rings for darkfield because it allows better control of intensity and direction of light.

Regards

Christian

[carlos.uruguay]

Very nice

[zzffnn]

Excellent images! Thank you for sharing.

[Smokedaddy]

Excellent!

[Olympusman]

Nice find and nice images.

Mike

[Jacek]

Super