Closterium, auto-fluorescence, stereo (red overload!)

Images made through a microscope. All subject types.

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Charles Krebs
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Closterium, auto-fluorescence, stereo (red overload!)

Post by Charles Krebs »

Image

Image

Image

These last three are not stereo. In the last image, lacy "tree-like" sections initially fluoresced much more than seen here, but they faded fast.

Image

Image

Image

Jacek
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Post by Jacek »

Super !

GaryB
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Post by GaryB »

That is crazy good.

I have no experience of fluorescence. Do you use a top light, fluor cube/dichoic filter and epi lens etc?

:?:

Charles Krebs
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Post by Charles Krebs »

GaryB wrote:I have no experience of fluorescence. Do you use a top light, fluor cube/dichoic filter and epi lens etc? :?:
Look through this thread to see how I set up:
http://www.photomacrography.net/forum/v ... hp?t=33123

In this case I am using external lights instead of an epi-illuminator. I use two different excitation wavelengths (~365nm UV and now ~455nm "Royal Blue") to illuminate the subject on the stage. The microscope has a "barrier filter" inserted that blocks the wavelengths emitted by the lights and only allows the longer wavelengths of the subject fluorescence to pass through. In basic principle, similar to the compound microscope fluorescence illuminators that use epi-illumination and various filter cubes. Chlorophyll auto-fluoresces as deep red under both of my light types.

GaryB
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Post by GaryB »

Thanks, Charles.

I love these DIY solutions, especially when the results are so good.

pwnell
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Post by pwnell »

Magnificent.

carlos.uruguay
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Post by carlos.uruguay »

Very nice as usual

fpelectronica
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Post by fpelectronica »

Very nice

curt0909
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Post by curt0909 »

Those stereo images are amazing! Great work

WalterD
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Post by WalterD »

Nice catch!

ChrisR
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Post by ChrisR »

Charles, have you seen significant difference, between the results from the two different excitation frequencies?
There may be a shift in the emission frequency, but if it's still overwhelmingly "red" to a sensor, then perhaps it would be indistinguishable.

Edit - I notice this was largely answered in a different thread. Easier to filter out the UV than the blue??
Chris R

Gerd
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Post by Gerd »

Hi Charly,
woaah, the stereos are absolutely stunning results with your setup, overwhelming indeed. With my filter-cube epi illumination there is a terrible blur all over the details, your images look like confocal images ! With your setup, do you have a visible red blur ? And another question: I guess, these are stacks ? Your image-processing is really perfect !
Kind regards,
Gerd

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