Closterium, auto-fluorescence, stereo (red overload!)
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- Charles Krebs
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Closterium, auto-fluorescence, stereo (red overload!)
These last three are not stereo. In the last image, lacy "tree-like" sections initially fluoresced much more than seen here, but they faded fast.
- Charles Krebs
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Look through this thread to see how I set up:GaryB wrote:I have no experience of fluorescence. Do you use a top light, fluor cube/dichoic filter and epi lens etc?
http://www.photomacrography.net/forum/v ... hp?t=33123
In this case I am using external lights instead of an epi-illuminator. I use two different excitation wavelengths (~365nm UV and now ~455nm "Royal Blue") to illuminate the subject on the stage. The microscope has a "barrier filter" inserted that blocks the wavelengths emitted by the lights and only allows the longer wavelengths of the subject fluorescence to pass through. In basic principle, similar to the compound microscope fluorescence illuminators that use epi-illumination and various filter cubes. Chlorophyll auto-fluoresces as deep red under both of my light types.
- carlos.uruguay
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Charles, have you seen significant difference, between the results from the two different excitation frequencies?
There may be a shift in the emission frequency, but if it's still overwhelmingly "red" to a sensor, then perhaps it would be indistinguishable.
Edit - I notice this was largely answered in a different thread. Easier to filter out the UV than the blue??
There may be a shift in the emission frequency, but if it's still overwhelmingly "red" to a sensor, then perhaps it would be indistinguishable.
Edit - I notice this was largely answered in a different thread. Easier to filter out the UV than the blue??
Chris R
Hi Charly,
woaah, the stereos are absolutely stunning results with your setup, overwhelming indeed. With my filter-cube epi illumination there is a terrible blur all over the details, your images look like confocal images ! With your setup, do you have a visible red blur ? And another question: I guess, these are stacks ? Your image-processing is really perfect !
Kind regards,
Gerd
woaah, the stereos are absolutely stunning results with your setup, overwhelming indeed. With my filter-cube epi illumination there is a terrible blur all over the details, your images look like confocal images ! With your setup, do you have a visible red blur ? And another question: I guess, these are stacks ? Your image-processing is really perfect !
Kind regards,
Gerd