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Snapshots From a Sphagnum Fen (Edited 10/03)

 
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WalterD



Joined: 06 Jul 2015
Posts: 233
Location: Rotterdam, the Netherlands

PostPosted: Wed Mar 08, 2017 10:54 pm    Post subject: Snapshots From a Sphagnum Fen (Edited 10/03) Reply with quote

My curiousity for what can be found in a drop of water from a mesotrophic (containing moderate amounts of nutrients) environment, led me late February -equipped with a plankton net- to one of the nearby sphagnum fens. Been there previously but still interested though about possible discoveries to be made this time of the year, end of the winter season.
And I was not being disappointed! Especially regarding micro-fauna it turned out to be crowded: Lots of different rotifers, including females with eggs were swarming underneath the coverslip. Furthermore ciliatea, amoebae, some cyclops and small water fleas (not the large ones that can be found more often). As expected concerning (micro-)flora it was less busy. In the samples I researched though, several species of desmids were found. A visit to another fen 3 months ago did not provide me any desmid that time. For the pictures I've brought some species together.
The characteristic Sphagnum, that creates an explosion of green each year was virtually absent, still I found one or two small pieces at the edge, under thin ice.

All and all a successful expedition. The funny thing is the mesotrophic water is in such a balance that it can easily be kept in a mini-aquarium outside for additional microscopic sessions...which is now duly noted.


1. Waterflea (Chydorus Sphaericus) DIC 25x


2. Rotifer (Gattung Keratella) Oil immersion polarisation 50x


3. Desmids (Closterium, Micrasterias and Euastrum) DIC 40x


4. Birch seed (betula) that ended up in the sample, 6,3x with compensator plate and mismatched phase stop.


5. Sphagnum brightfield 6.3x


6.Cyclops, "pseudo" DIC 6.3x


Click for double resolution on following Wetransfer link : https://we.tl/dZSi11vWNc
(expires 16.03.17)

Thanks for watching!
Cool


Last edited by WalterD on Fri Mar 10, 2017 4:59 am; edited 1 time in total
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Jacek



Joined: 02 Oct 2011
Posts: 4453
Location: Poland

PostPosted: Thu Mar 09, 2017 12:08 am    Post subject: Reply with quote

Very nice photos !
I am the nearest tank with acidic water to have more than 100 kilometers. It's my favorite environment due to desmids.
You in the Netherlands unless you have a lot of places. Envy Smile
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NikonUser



Joined: 04 Sep 2008
Posts: 2528
Location: southern New Brunswick, Canada

PostPosted: Thu Mar 09, 2017 5:35 am    Post subject: Reply with quote

Nice collection
#4 is a Birch seed Betula sp.
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student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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Beatsy



Joined: 05 Jul 2013
Posts: 801
Location: Malvern, UK

PostPosted: Thu Mar 09, 2017 6:44 am    Post subject: Reply with quote

Lovely set, but the rotifer is just stand-out stunning IMO. Thanks for sharing.
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Cactusdave



Joined: 09 Jun 2009
Posts: 1495
Location: Bromley, Kent, UK

PostPosted: Thu Mar 09, 2017 12:07 pm    Post subject: Reply with quote

Very nice. The rotifer is special. Shocked
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WalterD



Joined: 06 Jul 2015
Posts: 233
Location: Rotterdam, the Netherlands

PostPosted: Fri Mar 10, 2017 5:17 am    Post subject: Reply with quote

Good to hear that!
@ Jacek: "Nearby" still means a 1 hour drive in my case, anyway it's worth it.
@ Nikonuser: Thanks for the ID, saw you pictured a similar one a couple of years ago...already had this deja vu feeling... Smile
@ Beatsy & Cactusdave: Though not the expected first choice for examining plankton, I've been using this 50x/NA1,00 oil immersion objective for the Keratella (and for the water mite head in previous post). Of course it's a bit of luck as well, however the lens usually performs great, with excellent contrast and unexpected detail. I actually decided to buy one after reading very positive feedback in one of the objectives discussions in this forum.
7. Rotifer 25xDIC

8. Rotifer 40xDIC Compensator Plate (partially desaturated color-DIC)

9. Sphagnum 10x Darkfield

10. Sphagnum 16x Darkfield
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Jacek



Joined: 02 Oct 2011
Posts: 4453
Location: Poland

PostPosted: Sun Mar 12, 2017 12:25 pm    Post subject: Reply with quote

Sure it's worth, there are some beautiful microorganisms Very Happy

moss beautiful Very Happy
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Smokedaddy



Joined: 07 Oct 2006
Posts: 640
Location: Phoenix, Arizona

PostPosted: Sun Mar 12, 2017 4:56 pm    Post subject: Reply with quote

... excellent images, lots of work there too.
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 17793
Location: Richland, Washington State, USA

PostPosted: Sun Mar 12, 2017 5:57 pm    Post subject: Reply with quote

Marvelous work!

Are the rotifers single exposures or focus stacks?

--Rik
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WalterD



Joined: 06 Jul 2015
Posts: 233
Location: Rotterdam, the Netherlands

PostPosted: Mon Mar 13, 2017 11:51 am    Post subject: Reply with quote

Pleased you appreciate it.
Rik; all images, incl. the rotifers have been stacked, the majority with Zerene's D-map. The DIC I'm using (Smith-T) is confusing even the most sophisticated stacking software as it usually sees different details at different depths. This makes especially color-DIC time-consuming to process; you need to pick out the right details from each image manually.

To deal with this I've used only 5 to 10 images per picture and made separate small substacks, especially the top part as its continuously moving / retracting. After combining this, in photoshop more specific details from a single picture have sometimes been added by an additional layer combined with the history brush.

Cheers,

Walter
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GaryB



Joined: 29 Jul 2016
Posts: 357

PostPosted: Tue Mar 14, 2017 5:23 am    Post subject: Reply with quote

You have some beautiful colors going on in those shots.
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