Molds on decaying tangerine
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Molds on decaying tangerine
Just testing my fluorescence equipment for autofluorescence
Excitation Royal Blue 455nm LED. 10X Leitz Pl Fluotar 10/0.30 on Zeiss Standard. Stack of 77 images
Excitation Royal Blue 455nm LED. 10X Leitz Pl Fluotar 10/0.30 on Zeiss Standard. Stack of 77 images
Pau
Yes, it's classic epifluorescence with a filter cube, I used a bandpass green emission filter because all the sample was fluorescing in orangeish smearing the image.
Fluotar is the Leitz denomination for fluorite objectives, with blue excitation any other type would also work well, UV is more demanding about objective transmission.
Fluotar is the Leitz denomination for fluorite objectives, with blue excitation any other type would also work well, UV is more demanding about objective transmission.
Pau
Hi Walter,
I'm a beginner in fluorescence microscopy and I'm at the point of testing my LED setup with real subjects to take real images. I can't compare because I have no experience with HBO burners. I've bought the filters used and I'm not sure to have the right combinations in some cases
I'm very satisfied with blue LEDs, the former picture was taken at ISO 200 0.8s at 25X magnification on sensor with a 10/0.30 objective if these data can tell anything useful for you.
The main advantages of LED vs HBO are clear: no heat, instant response, much longer life (I expect), much less if any filter degradation, less dangerous...
The disadvantages I find are the low power of UV LEDs available and, with a DIY setup like mine, the need to change the LED for changing excitation and so multi spectral images will be more difficult to obtain. I'm convinced that HBO produce more intense UV radiation.
In few days (or weeks...) I will post my setup in the forum and I plan to keep posting some of my fluorescence images.
I'm a beginner in fluorescence microscopy and I'm at the point of testing my LED setup with real subjects to take real images. I can't compare because I have no experience with HBO burners. I've bought the filters used and I'm not sure to have the right combinations in some cases
I'm very satisfied with blue LEDs, the former picture was taken at ISO 200 0.8s at 25X magnification on sensor with a 10/0.30 objective if these data can tell anything useful for you.
The main advantages of LED vs HBO are clear: no heat, instant response, much longer life (I expect), much less if any filter degradation, less dangerous...
The disadvantages I find are the low power of UV LEDs available and, with a DIY setup like mine, the need to change the LED for changing excitation and so multi spectral images will be more difficult to obtain. I'm convinced that HBO produce more intense UV radiation.
In few days (or weeks...) I will post my setup in the forum and I plan to keep posting some of my fluorescence images.
Pau
Thanks for the interesting explanation, Pau. The advantages are indeed big, and diy approach is easier to carry out than with the HBO burners. Sounds like an interesting opportunity, the power of available UV leds might increase as well in the future. Looking forward to hear about your future posts!
Likewise, very interested in this
My extreme-macro.co.uk site, a learning site. Your comments and input there would be gratefully appreciated.
Jacek, thanks, interesting idea!
In fact with the Zeiss epifluorescence illuminator IV (the same you have mounted on the BIOLAR) the field is not full illuminated with the 4X objective, you can see some vignette at my first sorus image at the other post.
Be aware that working this way you're more exposed to UV radiation, even high intensity short blue can damage your eyes, I hope you wear UV protection glasses.
What models of UV LEDs do you use?
In fact with the Zeiss epifluorescence illuminator IV (the same you have mounted on the BIOLAR) the field is not full illuminated with the 4X objective, you can see some vignette at my first sorus image at the other post.
Be aware that working this way you're more exposed to UV radiation, even high intensity short blue can damage your eyes, I hope you wear UV protection glasses.
What models of UV LEDs do you use?
Pau
- carlos.uruguay
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Unfortunately, after Polish:
http://allegro.pl/dioda-mocy-power-led- ... 22213.html
Put a link to your setup because I can not find
http://allegro.pl/dioda-mocy-power-led- ... 22213.html
Put a link to your setup because I can not find