www.photomacrography.net :: View topic - Molds on decaying tangerine
www.photomacrography.net Forum Index
An online community dedicated to the practices of photomacrography, close-up and macro photography, and photomicrography.
Photomacrography Front Page Amateurmicrography Front Page
Old Forums/Galleries
 
 FAQFAQ   SearchSearch   MemberlistMemberlist   UsergroupsUsergroups   RegisterRegister 
 ProfileProfile   Log in to check your private messagesLog in to check your private messages   Log inLog in 
Molds on decaying tangerine
Goto page 1, 2  Next
 
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope
View previous topic :: View next topic  
Author Message
Pau
Site Admin


Joined: 20 Jan 2010
Posts: 4156
Location: Valencia, Spain

PostPosted: Sat Dec 10, 2016 1:18 pm    Post subject: Molds on decaying tangerine Reply with quote

Just testing my fluorescence equipment for autofluorescence
Excitation Royal Blue 455nm LED. 10X Leitz Pl Fluotar 10/0.30 on Zeiss Standard. Stack of 77 images


_________________
Pau
Back to top
View user's profile Send private message
ChrisR
Site Admin


Joined: 14 Mar 2009
Posts: 7504
Location: Near London, UK

PostPosted: Sat Dec 10, 2016 4:16 pm    Post subject: Reply with quote

Is this epi-lighting?
If not, I assume you wouldn't need a Fluotar?
_________________
Chris R
Back to top
View user's profile Send private message
Pau
Site Admin


Joined: 20 Jan 2010
Posts: 4156
Location: Valencia, Spain

PostPosted: Sat Dec 10, 2016 4:36 pm    Post subject: Reply with quote

Yes, it's classic epifluorescence with a filter cube, I used a bandpass green emission filter because all the sample was fluorescing in orangeish smearing the image.

Fluotar is the Leitz denomination for fluorite objectives, with blue excitation any other type would also work well, UV is more demanding about objective transmission.
_________________
Pau
Back to top
View user's profile Send private message
WalterD



Joined: 06 Jul 2015
Posts: 307
Location: Rotterdam, the Netherlands

PostPosted: Sun Dec 11, 2016 12:50 pm    Post subject: Reply with quote

Interesting image. Are you satisfied with use of leds on subject fluorescence microscope? I myself have been using a mercury vapor arc lamp, would be interesting to retrofit the illuminator with leds. What -do you think- are the most significant diffreneces between both (if any)?
Back to top
View user's profile Send private message Visit poster's website
Pau
Site Admin


Joined: 20 Jan 2010
Posts: 4156
Location: Valencia, Spain

PostPosted: Sun Dec 11, 2016 3:51 pm    Post subject: Reply with quote

Hi Walter,
I'm a beginner in fluorescence microscopy and I'm at the point of testing my LED setup with real subjects to take real images. I can't compare because I have no experience with HBO burners. I've bought the filters used and I'm not sure to have the right combinations in some cases

I'm very satisfied with blue LEDs, the former picture was taken at ISO 200 0.8s at 25X magnification on sensor with a 10/0.30 objective if these data can tell anything useful for you.
The main advantages of LED vs HBO are clear: no heat, instant response, much longer life (I expect), much less if any filter degradation, less dangerous...
The disadvantages I find are the low power of UV LEDs available and, with a DIY setup like mine, the need to change the LED for changing excitation and so multi spectral images will be more difficult to obtain. I'm convinced that HBO produce more intense UV radiation.

In few days (or weeks...) I will post my setup in the forum and I plan to keep posting some of my fluorescence images.
_________________
Pau
Back to top
View user's profile Send private message
WalterD



Joined: 06 Jul 2015
Posts: 307
Location: Rotterdam, the Netherlands

PostPosted: Mon Dec 12, 2016 4:59 am    Post subject: Reply with quote

Thanks for the interesting explanation, Pau. The advantages are indeed big, and diy approach is easier to carry out than with the HBO burners. Sounds like an interesting opportunity, the power of available UV leds might increase as well in the future. Looking forward to hear about your future posts!
Back to top
View user's profile Send private message Visit poster's website
johan



Joined: 06 Sep 2011
Posts: 971

PostPosted: Mon Dec 12, 2016 5:33 am    Post subject: Reply with quote

Likewise, very interested in this
_________________
My extreme-macro.co.uk site, a learning site. Your comments and input there would be gratefully appreciated.
Back to top
View user's profile Send private message Visit poster's website
Jacek



Joined: 02 Oct 2011
Posts: 4841
Location: Poland

PostPosted: Mon Dec 12, 2016 5:46 am    Post subject: Reply with quote

Pau, poor power LED UV 365 nm, can be strengthened. For the epi illumination is actually an understatement. When lenses to UV is pretty good. I will show you my solution for small lenses for 10X and less. Upload photos because my English is google translator Sad
_________________
https://photos.app.goo.gl/MV9IynUtBdaKkVj53
Back to top
View user's profile Send private message Send e-mail
Pau
Site Admin


Joined: 20 Jan 2010
Posts: 4156
Location: Valencia, Spain

PostPosted: Mon Dec 12, 2016 10:55 am    Post subject: Reply with quote

Quote:
I will show you my solution for small lenses for 10X and less.

Jacek, will be nice to see it!
_________________
Pau
Back to top
View user's profile Send private message
Jacek



Joined: 02 Oct 2011
Posts: 4841
Location: Poland

PostPosted: Mon Dec 12, 2016 2:24 pm    Post subject: Reply with quote

Illuminators made from the upper lens of the condenser (Zeiss, PZO).
Execution of primitive, but it works very well






_________________
https://photos.app.goo.gl/MV9IynUtBdaKkVj53
Back to top
View user's profile Send private message Send e-mail
Pau
Site Admin


Joined: 20 Jan 2010
Posts: 4156
Location: Valencia, Spain

PostPosted: Mon Dec 12, 2016 2:54 pm    Post subject: Reply with quote

Jacek, thanks, interesting idea!

In fact with the Zeiss epifluorescence illuminator IV (the same you have mounted on the BIOLAR) the field is not full illuminated with the 4X objective, you can see some vignette at my first sorus image at the other post.

Be aware that working this way you're more exposed to UV radiation, even high intensity short blue can damage your eyes, I hope you wear UV protection glasses.

What models of UV LEDs do you use?
_________________
Pau
Back to top
View user's profile Send private message
Jacek



Joined: 02 Oct 2011
Posts: 4841
Location: Poland

PostPosted: Mon Dec 12, 2016 3:09 pm    Post subject: Reply with quote

With me on Biolar it is good, maybe because I have a cap 1.25x.
Led 3W 365 nm.
Please show how LEDs have mounted at home and how they are changing.
_________________
https://photos.app.goo.gl/MV9IynUtBdaKkVj53
Back to top
View user's profile Send private message Send e-mail
carlos.uruguay



Joined: 23 Feb 2012
Posts: 4864
Location: Uruguay - Montevideo - America del Sur

PostPosted: Mon Dec 12, 2016 7:08 pm    Post subject: Reply with quote

Nice!!!!
Thanks for the explanation
Back to top
View user's profile Send private message Send e-mail Visit poster's website
Pau
Site Admin


Joined: 20 Jan 2010
Posts: 4156
Location: Valencia, Spain

PostPosted: Wed Dec 14, 2016 11:03 am    Post subject: Reply with quote

Jacek wrote:
Led 3W 365 nm.
Please show how LEDs have mounted at home and how they are changing.


Could you provide the link for your 3W 365 nm LED?

I've posted my fluorescence setup at the Equipment discussions forum
_________________
Pau
Back to top
View user's profile Send private message
Jacek



Joined: 02 Oct 2011
Posts: 4841
Location: Poland

PostPosted: Wed Dec 14, 2016 11:21 am    Post subject: Reply with quote

Unfortunately, after Polish:

http://allegro.pl/dioda-mocy-power-led-3w-uv-ultrafiolet-365-370nm-i6644022213.html

Put a link to your setup because I can not find
_________________
https://photos.app.goo.gl/MV9IynUtBdaKkVj53
Back to top
View user's profile Send private message Send e-mail
Display posts from previous:   
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope All times are GMT - 7 Hours
Goto page 1, 2  Next
Page 1 of 2

 
Jump to:  
You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot vote in polls in this forum


Powered by phpBB © 2001, 2005 phpBB Group