Hi all - i'm a commercial photographer trying to do some microscopy in our research lab. we've bought the camera adapter kit and had a couple of goes with really bad results so i'm looking for some advice.
for starters, am i using the right kit? we have a Meiji EMZ-8TR microscope and i'm attaching a canon 60d.
i can get images, but can't seem to get any part of the focal plane really sharp. is it just a case of practice and getting to know the kit or are there any setup details i could be missing?
any help would be really appreciated
best regards,
phil sheldon
complete beginner needing advice
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Is it like this - page 9?
http://microscopeinternational.com/manu ... manual.pdf
Does para 3 make sense, or any difference?
http://microscopeinternational.com/manu ... manual.pdf
Does para 3 make sense, or any difference?
Rotate the adjustment ring on the straight
tube so as to set the red indicator line on the
upper tube portion at the top edge of the lower
tube. Then fix in place with the clamp screw.
You now should be set correctly for optimum
conditions of simultaneous visual observation
and photography.
You may want to provide some more details about your subject and magnification.
Very thick subjects need image stacking (single shot won't produce sharp focus throughout when subject is thick).
Low contrast subjects (e.g., transparent crystals) may demand use of contrast enhancing techniques such as epi-dark field.
Stereo / macroscopes, in genetal, cannot very sharp images at high magnifications. Their ovjectives usually have low NA value ( resolution).
And I assume you manually focus your lens to infinity? And you provided diffused illumination sufficiently?
Very thick subjects need image stacking (single shot won't produce sharp focus throughout when subject is thick).
Low contrast subjects (e.g., transparent crystals) may demand use of contrast enhancing techniques such as epi-dark field.
Stereo / macroscopes, in genetal, cannot very sharp images at high magnifications. Their ovjectives usually have low NA value ( resolution).
And I assume you manually focus your lens to infinity? And you provided diffused illumination sufficiently?
Selling my Canon FD 200mm F/2.8 lens
Phil,
I think your camera is not parfocal with viewing eyepieces (camera was not focused correctly in that photo, even though microscope eyepieces were).
Please try to focus using camera screen alone. With some cameras, switching to video mode will zoom in further than the photo mode, allowing you to focus as best as you can (once you obtain best focus in video mode, switch back to photo mode and you should get a good focus).
I think your camera is not parfocal with viewing eyepieces (camera was not focused correctly in that photo, even though microscope eyepieces were).
Please try to focus using camera screen alone. With some cameras, switching to video mode will zoom in further than the photo mode, allowing you to focus as best as you can (once you obtain best focus in video mode, switch back to photo mode and you should get a good focus).
Selling my Canon FD 200mm F/2.8 lens