another marine dino in fluorescence

Images made through a microscope. All subject types.

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René
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Joined: Sun Oct 08, 2006 4:22 am

another marine dino in fluorescence

Post by René »

Hi guys, here's the marine dinoflagellate Alexandrium ostenfeldii, one of the toxic algae in the sea. It is stained with calcofluor, which is an optical brightener. It stains the cellulose theca, so the different plates can be determined for identification. The cell is about 55um diameter, the fuzzy dots are tiny chlorophytes out of focus.

Frontal view (stack of 18 images, 1 um apart)
Image

Top view (stack of 23 images, 1um apart)
Image

This is a series of images 0.66 um apart from a detail on the top (the apical pore complex)
ImageImageImageImageImageImage

And here's the corresponding stack with Helicon Focus:
Image
The stack could be a bit better, the first image of the stack shows a more clear inner line of the 'key hole' pore, but this is what Helicon Focus makes of it in auto-mode. Mind you, this is at the edge of resolution for this lens. The thick line of the key hole is about 0.5um thick, the distance between the thick and thin line is 0.7um. The calculated resolution of the lens used here is 0.5um with green light (20x/NA 0.7).

Best wishes, René

carlos.uruguay
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Post by carlos.uruguay »

Excellent!!

RogelioMoreno
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Post by RogelioMoreno »

Very nice details on #1.

Rogelio

Marek Mis
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Post by Marek Mis »

Very interesting,

Marek

Protos
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Post by Protos »

Nice fluo picture !
Thank you René !
Zeiss Axiophot, transmitted and Fluorescence
BK5000, Transmitted and CP
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piermicro
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Post by piermicro »

Very well.

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