A thin section by the needle of the Schwarzkiefer

Erik · Post by **Erik** » Fri Mar 30, 2007 7:10 am

Hello Microcopists,

I reads already one while with you also. After some days as registered participants comes now my first picture. This concerns a thin cut (20µ) by the needle of a Schwarzkiefer. The cut was colored following with Etzold.

I hope it find favours.

Many greetings Erik

rjlittlefield · Post by **rjlittlefield** » Fri Mar 30, 2007 7:55 am

Erik, welcome to the forums!

If I understand correctly, this is a cross section of the needle of a European Black Pine tree.

It is beautifully prepared and photographed -- great detail. How did you cut the section? What power and NA objectives did you use?

Thanks for posting -- we look forward to seeing many more!

--Rik

Erik · Post by **Erik** » Fri Mar 30, 2007 9:37 am

Hello Rik, thank you for the nice words.

"... How did you cut the section? ..."

I used an old microtome for students of E. Jung from Heidelberg (100 years old).

The needle of the Black Pine Tree is inserted first into AFE and been to to cut into elder Marks. AFE consists of alcohol, glacial acetic acid and Formalin (10: 1: 1).

"... What power and NA objectives did you use? ..."

I use an older microscope of Carl Zeiss Jena (earlier GDR, type Jenalab-Pol). For the first picture I used a 6,3x plan achromatic objective, for second a 50x plan apochromatic objective. Both photos free-handle taken up by the eyepiece with a Olympus C-40.

Erik

beetleman · Post by **beetleman** » Sat Mar 31, 2007 9:40 am

Wonderful pictures Erik, beautiful colors and excellent structures and shapes. Welcome to the forums Erik (extending hand out in greetings)

You have an excellent first post here and we hope to see many more

Ken Ramos · Post by **Ken Ramos** » Sat Mar 31, 2007 10:20 am

You guys with all this fancy cutlery, outstanding work Erik, beautifully done

and welcome to the forums.

Charles Krebs · Post by **Charles Krebs** » Sun Apr 01, 2007 3:42 pm

Erik... welcome!
As the other have said, beautiful preparation and images! It's looks like a wonderful abstract stained glass window.

Erik · Post by **Erik** » Sun Apr 01, 2007 11:39 pm

Thank you for the cordial welcome.

"... and we hope to see many more ..."

I will try it “class instead of mass” to deliver here.

Many greetings

Erik

my homepage:

http://www.mikroansichten.de