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Mosquito larva head, single & stero

 
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NikonUser



Joined: 04 Sep 2008
Posts: 2530
Location: southern New Brunswick, Canada

PostPosted: Mon Nov 12, 2012 12:33 pm    Post subject: Mosquito larva head, single & stero Reply with quote

I was surprised to see a few larvae in my outside aquarium. It seems unlikely that these will emerge as adults this year as the weather has turned cold (although very warm today, Nov 12 2012).
Larva in 50% Protoslo with a few drops of alcohol; fully sedated.
Ventral view of head capsule showing complex of mouthparts
10x Nikon CF N Plan Achromat on bellows, vertical setup, single flash, styrofoam cup diffuser, ZS Pmax.
single shot from jpg's
stereo from NEFs>TIFfs


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NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 18254
Location: Richland, Washington State, USA

PostPosted: Mon Nov 12, 2012 2:40 pm    Post subject: Reply with quote

Striking! Many thanks for the stereo. It makes the structure much more clear.

--Rik
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5762
Location: Issaquah, WA USA

PostPosted: Tue Nov 13, 2012 12:43 am    Post subject: Reply with quote

Very nice indeed!
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Cactusdave



Joined: 09 Jun 2009
Posts: 1523
Location: Bromley, Kent, UK

PostPosted: Tue Nov 13, 2012 1:34 am    Post subject: Reply with quote

Super!
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Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear
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Saul



Joined: 31 Jan 2011
Posts: 915
Location: Naperville, IL USA

PostPosted: Tue Nov 13, 2012 1:20 pm    Post subject: Reply with quote

Impressive photo !
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dissent



Joined: 10 Oct 2012
Posts: 144
Location: Illinois

PostPosted: Tue Nov 13, 2012 8:43 pm    Post subject: Reply with quote

I had no idea that mosquitoes has so much hair!! Terrific image.
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NikonUser



Joined: 04 Sep 2008
Posts: 2530
Location: southern New Brunswick, Canada

PostPosted: Tue Nov 13, 2012 8:50 pm    Post subject: Reply with quote

Thanks folks for your responses.
I cleared the head and made a permanent mount in Canada Balsam with the plan to show the mouth parts in more detail - but as noted by 'dissent' lots and lots of hair such that almost everything is obscured. Sad
_________________
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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NikonUser



Joined: 04 Sep 2008
Posts: 2530
Location: southern New Brunswick, Canada

PostPosted: Wed Nov 14, 2012 10:01 am    Post subject: Reply with quote

I did some more work on this mosquito larva, specifically the head+thorax to show imaginal discs and images of the siphon to show the characters useful for larval ID.
I decided to check and see what "Google" had to say about imaginal discs; surprise surprise, seems I posted similar info and images from what looks to be the same species almost 2 years ago.
The memory weakens.

http://www.photomacrography.net/forum/viewtopic.php?p=73385&sid=ffd11e4a40d63a70a1a19bf431ea97ef
_________________
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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ChrisR
Site Admin


Joined: 14 Mar 2009
Posts: 7268
Location: Near London, UK

PostPosted: Wed Nov 14, 2012 10:23 am    Post subject: Reply with quote

Quote:
permanent mount in Canada Balsam
Please 'scuse my ignorance, do you mean it's immersed in balsam, under a slide??
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NikonUser



Joined: 04 Sep 2008
Posts: 2530
Location: southern New Brunswick, Canada

PostPosted: Wed Nov 14, 2012 12:07 pm    Post subject: Reply with quote

The posted images, single shot & stereo of head, are of a live (heavily sedated, likely dead) larva in water+Protoslo+IPA.

Later I removed the head and mounted it, in Canada Balsam, on a slide under a coverglass. Such specimens become more transparent and more glass-like; better to see detail under a microscope.
_________________
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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curt0909



Joined: 26 Oct 2011
Posts: 607
Location: Pittsburgh, PA

PostPosted: Thu Nov 15, 2012 12:25 pm    Post subject: Reply with quote

Beautiful photo.
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lauriek
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Joined: 25 Nov 2007
Posts: 2404
Location: South East UK

PostPosted: Mon Nov 19, 2012 12:37 pm    Post subject: Re: Mosquito larva head, single & stero Reply with quote

NikonUser wrote:
Larva in 50% Protoslo with a few drops of alcohol; fully sedated.


Nicely shot and some good info here.

I remember when I shot one of these a while back I had it in a very small amount of water on a slide and it kept thrashing wildly around, had a great deal of trouble getting any stacks at all and was pleased to get anything I could post! Smile

Anyone know a good place to get Protoslo in the UK or Europe?
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NikonUser



Joined: 04 Sep 2008
Posts: 2530
Location: southern New Brunswick, Canada

PostPosted: Mon Nov 19, 2012 2:23 pm    Post subject: Reply with quote

Thanks curt & laurie.

Laurie, see here:
http://www.photomacrography.net/forum/viewtopic.php?t=14920
under Methyl Cellulose
_________________
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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View user's profile Send private message
dmillard



Joined: 24 Oct 2006
Posts: 501
Location: Austin, Texas

PostPosted: Tue Nov 20, 2012 3:07 pm    Post subject: Reply with quote

Beautiful images!
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