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Fern spore capsule and spore

 
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pwnell



Joined: 18 Dec 2009
Posts: 1861
Location: Tsawwassen, Canada

PostPosted: Sun Jul 22, 2012 1:55 pm    Post subject: Fern spore capsule and spore Reply with quote

Taken under brightfield, 20x * 2 magnification. 110 image stack using Helicon Focus with method A (Weighted Average). This was by far the better stack between it and Zerene Stacker for this subject, normally Zerene wins.



Last edited by pwnell on Sun Jul 22, 2012 8:57 pm; edited 1 time in total
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Pau
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Joined: 20 Jan 2010
Posts: 3567
Location: Valencia, Spain

PostPosted: Sun Jul 22, 2012 3:00 pm    Post subject: Reply with quote

Very nice!
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Cactusdave



Joined: 09 Jun 2009
Posts: 1481
Location: Bromley, Kent, UK

PostPosted: Mon Jul 23, 2012 8:40 am    Post subject: Reply with quote

Super image. It would make a great stereo pair. The potential sense of depth looks terrific.
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 17390
Location: Richland, Washington State, USA

PostPosted: Mon Jul 23, 2012 10:02 am    Post subject: Reply with quote

Waldo and I have been talking about this stack, and he graciously sent me a copy with permission to post about it. The stack has some really fascinating aspects, very different from stacks I've worked with before. There'll be much headscratching while I figure out what all is going on.

For stereo, that part is easy as long as we stick to PMax. Here's one I did last night while I was trying to be sure about the geometry. Crossed eye; the shifts are at +-2%.



--Rik
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 17390
Location: Richland, Washington State, USA

PostPosted: Mon Jul 23, 2012 10:51 am    Post subject: Reply with quote

Comparing the above PMax images against the Helicon Focus Method A image at top of thread, it's obvious that PMax has produced a much more "transparent" rendering of the structure. In the HF Method A image, the front surface of the capsule is rendered opaque -- we cannot see the spine of the capsule through the front surface in the rendering, even though obviously the scope can see through it in real life.

Producing an image like that with Zerene Stacker is not so easy. If we try to DMap the whole stack, then DMap gets confused in places and produces nasty "holes" where physically there aren't any. One fairly effective way around that problem is to do a Stack Selected DMap of just the front surface, then retouch that onto say a PMax of the whole stack, being careful to paint over only the parts we want to render opaque. Here's a stereo version of that approach:



As you can see, this stack presents some interesting artistic issues in addition to the optical and algorithmic issues that I'm still looking forward to studying. This stack is great fun!

--Rik
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5564
Location: Issaquah, WA USA

PostPosted: Tue Jul 24, 2012 2:18 am    Post subject: Reply with quote

Very nice image.

Definitely prefer the second stereo version... great to look at.
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Mitch640



Joined: 15 Aug 2010
Posts: 2137

PostPosted: Wed Jul 25, 2012 4:34 am    Post subject: Reply with quote

Yes, the last stereo image seems to be the best. It's an amazing find.
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